Fluorescence detection of trace PCB101 based on PITC immobilized on porous AAO membrane.
Fluorescence detection of trace PCB101 based on PITC immobilized on porous AAO membrane.
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DOI：
10.1039/c0an00510j
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Meiling Wang;G. Meng;Qing Huang;Ming-tao Li;Zhongbo Li;Chaolong Tang
中科院分区：
文献类型：
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作者：
Meiling Wang;G. Meng;Qing Huang;Ming-tao Li;Zhongbo Li;Chaolong Tang
将荧光团异硫氰酸苯酯（PITC）固定在多孔阳极氧化铝（AAO）膜上，制备了一种灵敏、选择性好的荧光膜（PITC@AAO），用于痕量2，2 '，4，5，5'-五氯联苯（PCB 101）的快速检测。当PCB 101滴入PITC@AAO膜后，PITC@AAO膜的荧光明显增强，这是由于荧光团PITC与PCB 101之间的卤素键合作用所致。PCB 101在1 ppm以下的低浓度范围内，荧光强度随PCB 101浓度的增加而增加，在1-6 ppb的低浓度范围内，荧光强度与PCB 101浓度之间存在近似的线性关系。此外，PITC@AAO膜显示出良好的选择性;例如，它对常见的结构类似物（多氯代芳族化合物）不敏感。对PITC@AAO膜对PCB 101的荧光增强机理以及PITC@AAO膜对PCB 101的灵敏度和选择性优于PITC/正己烷溶液的机理进行了讨论。这项工作表明，痕量（ppb范围内）的多氯联苯可以通过简单的荧光测量检测。
A sensitive and selective fluorescent membrane for rapid detection of trace 2,2',4,5,5'-pentachlorinated biphenyl (PCB101) has been achieved by immobilizing the fluorophore phenyl isothiocyanate (PITC) onto porous anodic aluminium oxide (AAO) membrane (denoted as PITC@AAO). The fluorescence of the PITC@AAO membrane is obviously enhanced after titrating the analyte PCB101 into the membrane, being ascribed to the halogen-bonding interaction between the fluorophore PITC and the analyte PCB101. The fluorescence intensity increases with the PCB101 concentration in the low range below 1 ppm, and there exists an approximate linear relationship between the relative fluorescence intensity and the PCB101 concentration in the low range of 1-6 ppb. Moreover, the PITC@AAO membrane shows good selectivity; for example, it is insensitive to common structural analogs (polychlorinated aromatics). The mechanisms of the fluorescence enhancement and the better sensitivity and selectivity of the PITC@AAO membrane to PCB101 than that of PITC/n-hexane solution are also discussed. This work demonstrates that trace (in ppb range) PCBs can be detected by simple fluorescence measurement.