A facile one-pot synthesis of polyethyleneimine functionalized graphene for the highly-sensitive and selective electrochemical impedance aptasensing of kanamycin in serum
A facile one-pot synthesis of polyethyleneimine functionalized graphene for the highly-sensitive and selective electrochemical impedance aptasensing of kanamycin in serum
复制标题
一种简便的一锅法合成聚乙烯亚胺功能化石墨烯，用于血清中卡那霉素的高灵敏度和选择性电化学阻抗适体传感
DOI：
10.1039/c9ay02120e
复制
发表时间：
2020-01-14
影响因子：
3.1
通讯作者：
Liu, Kunping
中科院分区：
文献类型：
--
作者：
Duan, Yuqing;Yang, Yuting;Liu, Kunping
采用聚乙烯亚胺（PEI）作为还原剂和功能化试剂，对剥离的氧化石墨烯（EGO）进行还原和包覆，使石墨烯保持单层状态，不发生聚集，从而实现了一锅法合成聚乙烯亚胺功能化石墨烯（PEI-G）。基于PEI-G良好的导电性和卡那霉素适体的高选择性，以PEI-G为电极基底增强电化学信号，以适体为生物识别单元提高选择性，制备了一种新型的电化学阻抗适配器，用于高灵敏度和高选择性检测血清中的卡那霉素。在特异性捕获样品中的卡那霉素后，所提出的适体传感器对卡那霉素在10 fM至100 pM的范围内表现出细线响应，检测限为5.2 fM。此外，该适体传感器还具有良好的重复性、稳定性和特异性，适用于真实的血清样品中痕量卡那霉素的分析。因此，这一建议的电化学阻抗适配策略打开了一个新的洞察卡那霉素的临床诊断使用的潜在应用。
A facile one-pot synthesis method for polyethyleneimine-functionalized graphene (PEI-G) was created by employing polyethyleneimine (PEI) as both the reductive and functionalized reagent to reduce and wrap exfoliated graphene oxide (EGO) to maintain the single layer status of graphene without aggregation. Based on the fine conductivity of PEI-G and high selectivity of the aptamer specially designed for kanamycin, a novel electrochemical impedance aptasensor for the highly-sensitive and selective detection of kanamycin in serum was fabricated using PEI-G as the electrode substrate to enhance the electrochemical signal and using the aptamer as a biological recognition unit to promote the selectivity. After the specific capture of kanamycin in the sample, the proposed aptasensor exhibited a fine line response for kanamycin within the range of 10 fM to 100 pM with a detection limit of 5.2 fM. Moreover, the proposed aptasensor also displayed a good reproducibility, stability and specificity, which was suitable for the trace kanamycin analyses in a real serum sample. Therefore, this proposed electrochemical impedance aptasensing strategy opens a new insight into potential applications in the clinical diagnostic use of kanamycin.