A protocol for membrane feeding assays to determine the infectiousness of P. falciparum naturally infected individuals to Anopheles gambiae.
A protocol for membrane feeding assays to determine the infectiousness of P. falciparum naturally infected individuals to Anopheles gambiae.
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DOI：
10.5281/zenodo.10926272
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发表时间：
2013-01-01
期刊：
影响因子：
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通讯作者：
Bousema, Teun
中科院分区：
文献类型：
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作者：
Ouedraogo, Andre Lin;Guelbeogo, Wamdaogo M;Bousema, Teun
在流行病学和临床研究中，蚊虫取食测定在量化疟疾传播潜力方面发挥着重要作用。目前，膜饲养试验还没有完全标准化。这影响了我们对检测方法精密度及其评价传播阻断干预措施适用性的理解。在这里，我们提出了一个详细的协议膜喂养冈比亚按蚊和自然恶性疟原虫感染的个人。
Mosquito feeding assays play an important role in quantifying malaria transmission potential in epidemiological and clinical studies. At present, membrane feeding assays are incompletely standardised. This affects our understanding of the precision of the assay and its suitability for evaluating transmission-blocking interventions. Here, we present a detailed protocol for membrane feeding using Anopheles gambiae mosquitoes and naturally P. falciparum infected individuals.