Production of a Monoclonal Antibody for C/EBPB : The Subnuclear Localization of C/EBPB in Mouse L929 Cells.
Production of a Monoclonal Antibody for C/EBPB : The Subnuclear Localization of C/EBPB in Mouse L929 Cells.
复制标题
C/EBPB 单克隆抗体的产生：小鼠 L929 细胞中 C/EBPB 的亚核定位。
DOI：
10.1089/mab.2013.0069
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Ohkawa Y.
中科院分区：
文献类型：
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作者：
Harada A;Okazaki E;Okada S;Tachibana T;Ohkawa Y.
CCAAT/增强子结合蛋白（C/EBP）β属于具有碱性亮氨酸拉链DNA结合结构域的C/EBP蛋白家族。这些蛋白质通过二聚化结合DNA，并在各种细胞的转录调控中发挥作用。存在六种不同类型的C/EBP，并且一些通过使用替代翻译起始位点形成同种型。因此，C/EBP家族的功能分析是难以实现的。本文报道用大鼠髂内淋巴结法制备抗小鼠C/EBPβ的特异性单克隆抗体。使用C/EBPβ单克隆抗体的免疫印迹鉴定了两种类型的亚型，而免疫染色显示C/EBPβ的亚核定位。该抗体的应用将有助于进一步阐明C/EBPβ的转录调控功能。
The CCAAT/enhancer-binding protein (C/EBP)β belongs to the C/EBP family of proteins that possesses a basic leucine zipper DNA-binding domain. These proteins bind DNA by dimerization and play a role in the transcriptional regulation of various cells. There are six different types of C/EBPs, and some form isoforms through the use of alternative translation initiation sites. The functional analysis of the C/EBP family is therefore difficult to achieve. Here we report on the production of specific monoclonal antibodies against mouse C/EBPβ using a rat medial iliac lymph node method. Immunoblotting using C/EBPβ monoclonal antibodies identified two types of isoforms, while immunostaining revealed a subnuclear localization for C/EBPβ. Use of this antibody should contribute to the further elucidation of the transcriptional regulatory function of C/EBPβ.