Group B streptococcal neuraminidase is actually a hyaluronidase.
Group B streptococcal neuraminidase is actually a hyaluronidase.
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B 族链球菌神经氨酸酶实际上是一种透明质酸酶。
DOI：
10.1128/iai.61.8.3234-3239.1993
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发表时间：
1993
影响因子：
3.1
通讯作者：
Lin,B
中科院分区：
文献类型：
--
作者：
Pritchard,DG;Lin,B
在许多报告中描述为神经氨酸酶的胞外B族链球菌酶实际上是透明质酸酶。在过去的25年中，酶进行了常规测定与牛颌下粘蛋白作为底物，并通过硫代巴比妥酸程序来测量释放的唾液酸。对酶释放的实际化合物的表征表明，它是透明质酸的α，β-不饱和衍生物，其来源于污染粘蛋白制剂的透明质酸。以前的报告描述了细胞外神经氨酸酶水平升高与B组链球菌的强毒株之间的关系，必须重新评估，认识到这种酶实际上是一种透明质酸酶。
The extracellular group B streptococcal enzyme described in numerous reports as a neuraminidase is really a hyaluronidase. Over the past 25 years, the enzyme was routinely assayed with bovine submaxillary mucin as the substrate and by the thiobarbituric acid procedure to measure released sialic acid. Characterization of the actual compound released by the enzyme revealed it to be an alpha,beta-unsaturated derivative of hyalobiuronic acid that was derived from hyaluronic acid contaminating the mucin preparation. Previous reports describing an association of elevated levels of extracellular neuraminidase with virulent strains of group B streptococci must be reevaluated with the recognition that the enzyme is really a hyaluronidase.