Resident Microbiota of the Gypsy Moth Midgut Harbors Antibiotic Resistance Determinants
Resident Microbiota of the Gypsy Moth Midgut Harbors Antibiotic Resistance Determinants
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DOI：
10.1089/dna.2008.0812
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发表时间：
2009-03-01
影响因子：
3.1
通讯作者：
Handelsman, Jo
中科院分区：
文献类型：
--
作者：
Allen, Heather K.;Cloud-Hansen, Karen A.;Handelsman, Jo
昆虫作为耐药细菌的环境储存库的重要性知之甚少。我们的特点是在舞毒蛾幼虫中肠的微生物群落的抗生素耐药基因组应用功能宏基因组培养的菌株。12种抗生素的最低抑菌浓度测定了44个培养的分离株，抗生素耐药基因的选择来自宏基因组文库提取的DNA从池的分离株。鉴定了六个独特的克隆。两个是高度耐青霉素型β-内酰胺类，两个是中度耐红霉素，两个是中度耐一系列抗生素，包括红霉素，羧苄青霉素，氯霉素。序列分析预测，活性基因编码外排泵，外排泵蛋白表达的转录激活因子，和超广谱A类β-内酰胺酶。昆虫肠道是抗生素抗性基因的储存库，具有传播的潜力。
Little is known about the significance of insects as environmental reservoirs of antibiotic-resistant bacteria. We characterized the antibiotic resistome of the microbial community in gypsy moth larval midguts by applying functional metagenomics to cultured isolates. The minimum inhibitory concentrations of 12 antibiotics were determined for 44 cultured isolates, and antibiotic resistance genes were selected from metagenomic libraries derived from DNA extracted from a pool of the isolates. Six unique clones were identified. Two were highly resistant to penicillin-type beta-lactams, two were moderately resistant to erythromycin, and two were moderately resistant to a range of antibiotics, including erythromycin, carbenicillin, and chloramphenicol. Sequence analysis predicted that the active genes encoded efflux pumps, a transcriptional activator of efflux pump protein expression, and an extended-spectrum class A beta-lactamase. Insect guts are a reservoir of antibiotic resistance genes with the potential for dissemination.