Isolation of bacterial strains able to degrade biphenyl, diphenyl ether and the heat transfer fluid used in thermo-solar plants
Isolation of bacterial strains able to degrade biphenyl, diphenyl ether and the heat transfer fluid used in thermo-solar plants
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DOI：
10.1016/j.nbt.2016.11.003
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发表时间：
2017-03-25
影响因子：
5.4
通讯作者：
Moreno-Vivian, Conrado
中科院分区：
文献类型：
--
作者：
Blanco-Moreno, Rafael;Saez, Lara P.;Moreno-Vivian, Conrado
热电厂使用二苯醚（DE）和联苯（BP）的低共熔混合物作为传热流体（HTF）。HTF的潜在损失可能会污染土壤，生物修复是一种有吸引力的处理工具。DE-或BP-降解细菌是已知的，但迄今为止尚未描述能够降解HTF混合物的细菌。在这里，五个细菌菌株，能够生长与HTF或其单独的组件DE和BP作为唯一的碳源已被分离，无论是从暴露于HTF的土壤或从根际土壤的植物生长附近的热太阳能发电厂。通过16 S rRNA基因测序将微生物鉴定为皮肖无色杆菌菌株BioC 1、杀多糖假单胞菌菌株6.1、铜绿假单胞菌菌株HBD 1和HBD 3以及食油假单胞菌菌株HBD 2。在所有菌株中检测到联苯上游降解途径的关键酶2，3-二羟基联苯双加氧酶（BphC）的活性。假单胞菌菌株几乎完全降解2000 ppm的HTF培养5天后，甚至耐受和生长在150，000 ppm的HTF的存在下，是合适的候选人在原位土壤生物修复。HTF的两种组分的降解是特别感兴趣的，因为在DE-降解剂鞘氨醇单胞菌属SS 3中，DE或苯甲酸盐的生长受到BP的强烈抑制。(C)© 2016 Elsevier B. V.版权所有。
Thermo-solar plants use eutectic mixtures of diphenyl ether (DE) and biphenyl (BP) as heat transfer fluid (HTF). Potential losses of HTF may contaminate soils and bioremediation is an attractive tool for its treatment. DE-or BP-degrading bacteria are known, but up to now bacteria able to degrade HTF mixture have not been described. Here, five bacterial strains which are able to grow with HTF or its separate components DE and BP as sole carbon sources have been isolated, either from soils exposed to HTF or from rhizospheric soils of plants growing near a thermo-solar plant. The organisms were identified by 16S rRNA gene sequencing as Achromobacter piechaudii strain BioC1, Pseudomonas plecoglossicida strain 6.1, Pseudomonas aeruginosa strains HBD1 and HBD3, and Pseudomonas oleovorans strain HBD2. Activity of 2,3-dihydroxybiphenyl dioxygenase (BphC), a key enzyme of the biphenyl upper degradation pathway, was detected in all isolates. Pseudomonas strains almost completely degraded 2000 ppm HTF after 5-day culture, and even tolerated and grew in the presence of 150,000 ppm HTF, being suitable candidates for in situ soil bioremediation. Degradation of both components of HTF is of particular interest since in the DE-degrader Sphingomonas sp. SS3, growth on DE or benzoate was strongly inhibited by addition of BP. (C) 2016 Elsevier B.V. All rights reserved.