Tensin3 is a novel thyroid-specific gene
Tensin3 is a novel thyroid-specific gene
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DOI：
10.1677/jme.1.01913
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发表时间：
2006-02-01
影响因子：
3.5
通讯作者：
Miyauchi, A
中科院分区：
文献类型：
--
作者：
Maeda, I;Takano, T;Miyauchi, A
甲状腺特异性基因，如甲状腺过氧化物酶、甲状腺球蛋白、Na+/I-同调体和促甲状腺激素受体等，在甲状腺功能中起着重要作用，并与许多病理状况有关。通过序列特异性差异显示，我们检测到三个基因在正常甲状腺组织中的表达水平高于甲状腺肿瘤组织。经过压制和测序分析，发现其中一个基因为tensin3。实时荧光定量PCR检测tensin3的表达水平。其在甲状腺肿瘤组织中的表达水平明显低于正常甲状腺组织。在两种间变性癌中，这种下降更为明显。在甲状腺和胎盘中分别观察到高水平和中等水平的tensin3mrna表达。tensin3mrna仅在脑、心、肺、肝、胰腺、肾、骨骼肌、白细胞和前列腺等其他组织中低水平表达。这些结果表明，tensin3是一个新的甲状腺特异性基因，进一步的研究可能揭示其与甲状腺功能或甲状腺疾病的关系。
Thyroid-specific genes, such as thyroid peroxidase, thyroglobulin, Na+/I- symporter and thyroid-stimulating hormone receptor, play fundamental roles in thyroid function and relate to many pathological conditions. Using sequence specific-differential display, we detected three genes that showed higher expression levels in normal thyroid tissues than in thyroid tumor tissues. After subdoning and sequencing analysis, one of the genes was revealed to be tensin3. The expression level of tensin3 was examined with real-time quantitative PCR analysis. Its expression levels were more depressed in thyroid tumor tissues than in normal thyroid tissues. The decrease was even more evident in two anaplastic carcinomas. High and moderate levels of tensin3 mRNA expression were observed in the thyroid and placenta respectively. Tensin3 mRNA was expressed only in low levels in other tissues, such as the brain, heart, lung, liver, pancreas, kidney, skeletal muscle, white blood cells and prostate. These results show that tensin3 is a novel thyroid-specific gene and further investigations may reveal its relation to thyroid function or thyroid disease.