PARTITIONING OF BACTERIAL PLASMIDS DURING CELL-DIVISION - A CIS-ACTING LOCUS THAT ACCOMPLISHES STABLE PLASMID INHERITANCE
PARTITIONING OF BACTERIAL PLASMIDS DURING CELL-DIVISION - A CIS-ACTING LOCUS THAT ACCOMPLISHES STABLE PLASMID INHERITANCE
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DOI：
10.1016/0092-8674(80)90639-x
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
COHEN, SN
中科院分区：
文献类型：
--
作者：
MEACOCK, PA;COHEN, SN
鉴定并表征了在指数生长的细胞群体内稳定维持质粒所需的遗传功能（指定为par，用于分区）。该功能实现质粒DNA分子向子细胞的主动分布，其定位于pSC 101质粒内邻近复制起点的270 bp片段。似乎与真核细胞的着丝粒在功能上等同的par基因座能够拯救不稳定的pSC 101衍生的复制子或不相关的par-P15 A衍生的顺式但非反式构型的多拷贝复制子。它不依赖于拷贝数控制，也不指定质粒不相容性。它不直接与质粒复制功能相关。
A genetic function (designated par, for partition) was identified and characterized that is required for stable maintenance of plasmids within exponentially growing cell populations. This function, which accomplishes the active distribution of plasmid DNA molecules to daughter cells, was localized within the pSC101 plasmid to a 270 bp segment adjacent to the replication origin. The par locus, which appears to be functionally equivalent to the centromere of eukaryotic cells, is able to rescue unstable pSC101-derived replicons or an unrelated par- P15A-derived multicopy replicon in the cis, but not the trans, configuration. It is independent of copy number control and does not specify plasmid incompatibility. It is not associated directly with plasmid replication functions.