Mouse Models of Tumor Bone Metastasis and Invasion for Studying CCN Proteins.
Mouse Models of Tumor Bone Metastasis and Invasion for Studying CCN Proteins.
复制标题
用于研究 CCN 蛋白的肿瘤骨转移和侵袭的小鼠模型。
DOI：
10.1007/978-1-0716-2744-0_24
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Sasaki A.
中科院分区：
文献类型：
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作者：
Shimo T;Okui T;Horie N;Yokozeki K;Takigawa M;Sasaki A.
骨转移和骨破坏是人类常见的恶性肿瘤，包括乳腺癌、前列腺癌和肺癌，由于顽固性骨痛、病理性骨折、高钙血症和神经压迫，骨转移和骨破坏发生率高。骨转移和骨破坏的动物模型是研究其发病机制和制定治疗策略的重要工具。然而，尽管动物经常自发地患上癌症，但很少有自发骨转移的模型。在这里，我们描述了建立乳腺癌骨转移小鼠模型的方法，该模型是通过将MDA-MB-231乳腺癌细胞注射到左心室来实现的。此外，我们还介绍了将口腔鳞癌细胞注射到右侧胫骨干骺端骨髓腔内造成骨破坏的小鼠模型。这些检测方法可用于研究细胞通讯网络因子/结缔组织生长因子(CTGF/CCN2)蛋白在肿瘤转移中的作用以及开发针对CCN蛋白的治疗策略。
Bone metastasis and bone destruction are common occurrences in human malignancies, including breast, prostate, and lung cancer, and are associated with a high morbidity rate because of intractable bone pain, pathological fractures, hypercalcemia, and nerve compression. Animal models of bone metastasis and bone destruction are important tools to investigate the pathogenesis and develop treatment strategies. However, there are few models of spontaneous bone metastasis despite the fact that animals often spontaneously develop cancer. Here, we describe methods for developing a mouse model of breast cancer bone metastasis achieved by injection of MDA-MB-231 breast cancer cells into the left cardiac ventricle. In addition, we introduce mouse model of the bone destruction by injection of SAS oral squamous cell carcinoma cells into the bone marrow space of the right tibial metaphysis. These assays can be applied to studies on roles of cellular communication network factor/connective tissue growth factor (CTGF/CCN2) protein in tumor metastasis and development of treatment strategies targeting CCN proteins.