Connexin expression by radial glia-like cells is required for neurogenesis in the adult dentate gyrus
Connexin expression by radial glia-like cells is required for neurogenesis in the adult dentate gyrus
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DOI：
10.1073/pnas.0813160106
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发表时间：
2009-07-07
影响因子：
11.1
通讯作者：
Steinhaeuser, Christian
中科院分区：
文献类型：
--
作者：
Kunze, Albrecht;Congreso, Marga Rubenecia;Steinhaeuser, Christian
在成年齿状回中，放射状胶质样细胞代表产生神经元和胶质细胞的假定干细胞。在这里，我们结合膜片钳记录，生物素填充，免疫组织化学，单细胞转录分析，和小鼠转基因测试连接蛋白的表达和缝隙连接耦合放射状胶质样细胞及其对神经发生的影响。在巢蛋白和gfap启动子控制下表达EGFP的小鼠中鉴定出放射状胶质样细胞。我们发现，大多数放射状胶质样细胞是耦合和表达Cx43。神经元前体不偶联。在GFAP阳性细胞中缺乏Cx30和Cx43的小鼠表现出几乎完全的增殖抑制和放射状胶质样细胞和颗粒神经元数量的显著下降。诱导型病毒介导的成年海马连接蛋白的消融也减少了神经发生。这些研究结果强烈建议的要求连接蛋白表达的放射状胶质样细胞完整的神经发生在成人大脑中，并指出这些细胞可能的通信途径。
In the adult dentate gyrus, radial glia-like cells represent putative stem cells generating neurons and glial cells. Here, we combined patch-clamp recordings, biocytin filling, immunohistochemistry, single-cell transcript analysis, and mouse transgenics to test for connexin expression and gap junctional coupling of radial glia-like cells and its impact on neurogenesis. Radial glia-like cells were identified in mice expressing EGFP under control of the nestin and gfap promoters. We show that a majority of Radial glia-like cells are coupled and express Cx43. Neuronal precursors were not coupled. Mice lacking Cx30 and Cx43 in GFAP-positive cells displayed almost complete inhibition of proliferation and a significant decline in numbers of radial glia-like cells and granule neurons. Inducible virus-mediated ablation of connexins in the adult hippocampus also reduced neurogenesis. These findings strongly suggest the requirement of connexin expression by radial glia-like cells for intact neurogenesis in the adult brain and point to possible communication pathways of these cells.