Identification and chromosomal localization of CTNNAL1, a novel protein homologous to α-catenin
Identification and chromosomal localization of CTNNAL1, a novel protein homologous to α-catenin
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DOI：
10.1006/geno.1998.5458
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发表时间：
1998-11-15
期刊：
影响因子：
4.4
通讯作者：
Smith, DI
中科院分区：
文献类型：
--
作者：
Zhang, JS;Nelson, M;Smith, DI
丁酸钠（NaB）可诱导人胰腺癌细胞株（AsPC-1）的生长抑制和凋亡。为了鉴定由NaB差异调节的基因，我们使用抑制消减杂交技术产生高度富集上调或下调转录物的消减EST文库。在下调文库中鉴定的EST之一显示出与人α-连环蛋白的显著同源性。克隆了一个2.45kb的cDNA，编码734个氨基酸的蛋白质。将克隆的cDNA用作体外转录-翻译反应中的模板。这产生了大小为约82 kDa的蛋白质产物。被命名为CTNNAL 1（连环蛋白（钙粘蛋白相关蛋白），α样1）的基因被发现在许多组织中普遍表达，包括胰腺，心脏和骨骼肌。分离含有该基因的人BAC克隆并用作荧光原位杂交（FISH）的探针。放射杂交和FISH分析均将该基因定位于染色体带9q31.2，该区域在膀胱癌、食管癌和其他几种肿瘤中经常观察到异常，（C）1998 Academic Press。
Sodium butyrate (NaB) was shown to induce growth inhibition and apoptosis in a human pancreatic cancer cell line (AsPC-1). To identify the genes that are differentially regulated by NaB, we generated subtracted EST libraries highly enriched for up- or down-regulated transcripts using the suppression subtraction hybridization technique. One of the ESTs identified in the down-regulated library showed significant homology to human alpha-catenin. A cDNA of 2.45 kb that codes for a putative protein of 734 aa was cloned. The cloned cDNA was used as a template in an in vitro transcription-translation reaction. This produced a protein product of about 82 kDa in size. The gene designated as CTNNAL1 (catenin (cadherin-associated protein), alpha-like 1) was found to be ubiquitously expressed in many tissues including pancreas, heart, and skeletal muscle. A human BAC clone containing the gene was isolated and used as a probe for fluorescence in situ hybridization (FISH). Both radiation hybrid and FISH analysis mapped the gene to chromosome band 9q31.2, a region where frequent abnormalities have been observed in bladder carcinoma, esophageal cancers, and several other tumors, (C) 1998 Academic Press.