trans-spliceosomal U6 RNAs of Crithidia fasciculata and Leptomonas seymouri: deviation from the conserved ACAGAG sequence and potential base pairing with spliced leader RNA

trans-spliceosomal U6 RNAs of Crithidia fasciculata and Leptomonas seymouri: deviation from the conserved ACAGAG sequence and potential base pairing with spliced leader RNA
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Crithidia fasciculata 和 Leptomonas seymouri 的反式剪接体 U6 RNA:与保守 ACAGAG 序列的偏差以及与剪接前导 RNA 的潜在碱基配对

DOI:
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发表时间:
1994
影响因子:
5.3
通讯作者:
Albrecht Bindereif
Albrecht Bindereif
中科院分区:
生物学2区
文献类型:
--
作者:
Guoliang Xu;Björn Wieland;Albrecht Bindereif

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分离得到了束状锥虫和西摩瘦单胞菌的U6 RNA基因。与布鲁氏锥虫一样,束状锥虫和西摩氏锥虫的U6 RNA基因与上游tRNA基因紧密连锁。束状棘球蚴(c.a fasciculata)和西摩棘球蚴(l.s seymouri)的U6 RNA序列与已发表的布氏绦虫序列分别有5个和3个位置的差异。有趣的是,在ACAGAG六核苷酸序列的第2位,两种束草U6 RNA基因都携带C- >T突变,这对剪接功能很重要,并且被认为是系统进化上不变的。在高度保守的5‘剪接位点+5,G- >A处,C. fasciculata剪接先导RNA的代偿性碱基变化表明,最近提出的酵母顺式剪接系统的5’剪接位点区域与U6 RNA之间的相互作用也可能发生在反式剪接中。
U6 RNA genes from the trypanosomatids Crithidia fasciculata and Leptomonas seymouri have been isolated and sequenced. As in Trypanosoma brucei, the U6 RNA genes in both C. fasciculata and L. seymouri are arranged in close linkage with upstream tRNA genes. The U6 RNA sequences from C. fasciculata and L. seymouri deviate in five and three positions, respectively, from the published T. brucei sequence. Interestingly, both C. fasciculata U6 RNA genes carry a C-->T change at the second position of the ACAGAG hexanucleotide sequence, which is important for splicing function and has been considered phylogenetically invariable. A compensatory base change of the C. fasciculata spliced leader RNA at the highly conserved 5' splice site position +5, G-->A, suggests that an interaction between the 5' splice site region and U6 RNA recently proposed for the yeast cis-splicing system may also occur in trans splicing.
Leptomonas seymouri 作为锥虫基因表达分析的模型系统。
DOI: --
发表时间: 1993
期刊: The Journal of parasitology
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