Potency of Transgenic Effectors for Neurogenetic Manipulation in Drosophila Larvae
Potency of Transgenic Effectors for Neurogenetic Manipulation in Drosophila Larvae
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DOI：
10.1534/genetics.114.172023
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发表时间：
2014-10
期刊：
影响因子：
3.3
通讯作者：
D. Pauls;Alina von Essen;Radostina Lyutova;Lena van Giesen;Ronny Rosner;C. Wegener;S. Sprecher
中科院分区：
文献类型：
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作者：
D. Pauls;Alina von Essen;Radostina Lyutova;Lena van Giesen;Ronny Rosner;C. Wegener;S. Sprecher
神经元活动的基因操作是旨在确定特定神经元对动物行为的功能影响的研究的基石。果蝇因其神经系统小、生命周期快和遗传适应性强，为研究神经元回路功能提供了一个有吸引力的模型系统。在过去的二十年里，人们开发出了大量优雅的遗传工具来操纵和研究果蝇的神经回路。目前的技术允许基因消融、组成性沉默或神经元活动过度激活，还包括条件性热遗传学或光遗传学激活或抑制。对于所有遗传技术来说，选择合适的转基因工具对于行为研究至关重要。效应器的效力和影响可能因不同的神经元类型或不同的行为类型而异。我们在这里系统地测试遗传效应器改变果蝇幼虫行为的效力，使用两种不同的行为范式：一般运动活动和定向、视觉引导导航。我们的结果显示，两种测定中不同效应细胞系的效果基本相似但不相等。有趣的是，两种行为测定之间不同效应系之间诱导的行为改变程度的差异在很大程度上保持一致。这里评估的胺能和胆碱能神经元中观察到的效应线的效力可能有助于研究人员选择最合适的遗传工具来剖析果蝇幼虫行为背后的神经元网络。
Genetic manipulations of neuronal activity are a cornerstone of studies aimed to identify the functional impact of defined neurons for animal behavior. With its small nervous system, rapid life cycle, and genetic amenability, the fruit fly Drosophila melanogaster provides an attractive model system to study neuronal circuit function. In the past two decades, a large repertoire of elegant genetic tools has been developed to manipulate and study neural circuits in the fruit fly. Current techniques allow genetic ablation, constitutive silencing, or hyperactivation of neuronal activity and also include conditional thermogenetic or optogenetic activation or inhibition. As for all genetic techniques, the choice of the proper transgenic tool is essential for behavioral studies. Potency and impact of effectors may vary in distinct neuron types or distinct types of behavior. We here systematically test genetic effectors for their potency to alter the behavior of Drosophila larvae, using two distinct behavioral paradigms: general locomotor activity and directed, visually guided navigation. Our results show largely similar but not equal effects with different effector lines in both assays. Interestingly, differences in the magnitude of induced behavioral alterations between different effector lines remain largely consistent between the two behavioral assays. The observed potencies of the effector lines in aminergic and cholinergic neurons assessed here may help researchers to choose the best-suited genetic tools to dissect neuronal networks underlying the behavior of larval fruit flies.