Phosphoserine Phosphatase Is a Novel Prognostic Biomarker on Chromosome 7 in Colorectal Cancer
Phosphoserine Phosphatase Is a Novel Prognostic Biomarker on Chromosome 7 in Colorectal Cancer
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DOI：
10.21873/anticanres.11574
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发表时间：
2017-05-01
影响因子：
2
通讯作者：
Mimori, Koshi
中科院分区：
文献类型：
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作者：
Sato, Kuniaki;Masuda, Takaaki;Mimori, Koshi
背景/目的：染色体7 p（Ch.7p）扩增在结直肠癌（CRC）中很常见。本研究的目的是鉴定Ch.7p上由于DNA拷贝数扩增而过表达的潜在驱动基因，并确定其在CRC中的临床意义。材料与方法：我们使用来自癌症基因组图谱（TCGA）的CRC数据集，使用生物信息学方法将磷酸丝氨酸磷酸酶（PSPH）确定为潜在的驱动基因。应用定量逆转录聚合酶链反应（PCR）和免疫组织化学方法检测124例原发性CRC中PSPH的表达。使用TCGA数据集通过基因集富集分析（GSEA）探索PSPH表达的生物学效应。结果：PSPH在肿瘤组织中呈高表达，且与肿瘤浸润深度和远处转移呈正相关。多因素分析显示，PSPH高表达是一个独立的不良预后因素。这些结果得到了GSEA的支持。结论：PSPH可能是一种新的预测大肠癌Ch.7p恶性潜能的生物标志物。
Background/ Aim: Amplification of chromosome 7p (Ch. 7p) is common in colorectal cancer (CRC). The aim of this study was to identify potential driver genes on Ch. 7p that are overexpressed due to DNA copy number amplification and determine their clinical significance in CRC. Materials and Methods: We identified phosphoserine phosphatase (PSPH) as a potential driver gene using a CRC dataset from The Cancer Genome Atlas (TCGA) using a bioinformatics approach. The expression of PSPH in 124 primary CRCs was examined by quantitative reverse transcription polymerase chain reaction (PCR) and immunohistochemistry. The biological effect of PSPH expression was explored by Gene Set Enrichment Analysis (GSEA) using the TCGA dataset. Results: PSPH was overexpressed in tumor tissues and PSPH positively correlated with depth of invasion and distant metastasis. On multivariate analysis, high PSPH expression was an independent poor prognostic factor. These results were supported by GSEA. Conclusion: PSPH could be a novel prognostic biomarker with malignant potential on Ch. 7p in CRC.