Pathogenic and humoral immune responses to porcine reproductive and respiratory syndrome virus (PRRSV) are related to viral load in acute infection
Pathogenic and humoral immune responses to porcine reproductive and respiratory syndrome virus (PRRSV) are related to viral load in acute infection
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DOI：
10.1016/j.vetimm.2004.09.010
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发表时间：
2004-12-08
影响因子：
1.8
通讯作者：
Murtaugh, MP
中科院分区：
文献类型：
--
作者：
Johnson, W;Roof, M;Murtaugh, MP
虽然人们对猪繁殖与呼吸综合征病毒(PRRSV)进行了大量的研究，但关于病毒毒力与体内病毒复制之间的关系、在PRRS历史上不同时间恢复的分离株之间的关系以及单个病毒分离株的相对毒力水平等方面的定量信息很少。本研究比较了PRRSV强毒株和弱毒株在体内的生长特性。结果表明，在呼吸道疾病模型中，强毒株表现出更长时间和更高水平的病毒血症，诱导更快和更强烈的体液免疫反应，对体重增加产生负面影响，诱导更高的死亡率，并导致更严重的临床症状。我们发现，毒力更强的田间分离株在猪身上的水平明显高于细胞培养适应株。我们的结论是，猪对PRRSV的致病后果和免疫反应与急性感染时的病毒载量直接相关，这反映在血液中的病毒滴度上。(C)2004爱思唯尔B.V.保留所有权利。
Although much research has been performed on porcine reproductive and respiratory syndrome virus (PRRSV), little quantitative information is available on the relationships between virulence and in vivo virus replication, among isolates recovered at different times in the history of PRRS, or the relative levels of virulence associated with individual virus isolates. In this study, the in vivo growth properties of virulent field isolates and attenuated PRRSV isolates were compared. The results show that virulent PRRSV isolates exhibit longer and more elevated levels of viremia, induce faster and more intense humoral immune responses, negatively affect body weight gain, induce higher death rates, and cause more severe clinical signs in a respiratory disease model. We found that the more virulent field isolates grew to significantly higher levels in pigs than did cell-culture adapted isolates. We concluded that the pathogenic consequences and immunological responses of pigs to PRRSV are directly related to viral load in acute infection as reflected in viral titers in blood. (C) 2004 Elsevier B.V. All rights reserved.