Optoacoustic Imaging of Naphthalocyanine: Potential for Contrast Enhancement and Therapy Monitoring
Optoacoustic Imaging of Naphthalocyanine: Potential for Contrast Enhancement and Therapy Monitoring
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DOI：
10.2967/jnumed.114.147157
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发表时间：
2015-02-01
影响因子：
9.3
通讯作者：
Ntziachristos, Vasilis
中科院分区：
文献类型：
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作者：
Beziere, Nicolas;Ntziachristos, Vasilis
我们在体外和体内研究了硅2，3-萘二（三己基甲硅烷基氧化物）（SiNc）的光声响应，SiNc在本文中被认为是用于光声成像的报告分子，阐明了其光声（光声）信号产生的效率并检查了所实现的体内性能。方法：使用Cremophor EL在水中制备SiNc溶液，并评价其光吸收和光声对比度产生特性。在脉冲激光照射下研究了光稳定性和单线态氧的产生，并使用光吸光度进行了验证。HT-29小鼠肿瘤模型用于评估化合物的生物分布及其作为体内光声造影剂的性能。结果：与常用的吲哚菁绿色相比，发现SiNc产生上级光声信号。小鼠肿瘤的多光谱光声断层扫描有效地解决了体内SiNc的生物分布和基础灌注参数。此外，我们演示了如何光引发的氮化硅与分子氧的反应可以潜在地感测，并讨论这些测量的光热处理中所涉及的生化过程的关系。结论：SiNc是一种很有前途的光声成像造影剂。进一步发展的可能性承诺扩大其在纯粹的对比生成设置中的使用，以及其光动力治疗应用。
We investigated in vitro and in vivo the optoacoustic responses of silicon 2,3-naphthalocyanine bi(trihexylsilyloxide) (SiNc), considered herein as a reporter molecule for optoacoustic imaging, elucidating its efficiency for optoacoustic (photoacoustic) signal generation and examining the in vivo performance achieved. Methods: SiNc solutions were prepared using Cremophor EL in water and evaluated for light-absorbing and optoacoustic contrast-generating properties. Photostability and singlet oxygen generation were investigated under pulsed laser illumination and validated using photoabsorbance. HT-29 mouse tumor models were used to assess the biodistribution of the compound and its performance as an optoacoustic contrast agent in vivo. Results: SiNc was found to generate superior optoacoustic signals compared with the commonly used indocyanine green. Multispectral optoacoustic tomography of mouse tumors efficiently resolved the biodistribution of SiNc and the underlying perfusion parameters in vivo. In addition, we demonstrate how light-triggered SiNc reactions with molecular oxygen can be potentially sensed and discuss the relation of these measurements to the biochemical process involved in photothermal treatment. Conclusion: SiNc appears to be a promising family of contrast agent for optoacoustic imaging. Further development possibilities promise to expand its use in purely contrast generation settings, as well as its photodynamic therapy application.