A Simple and Rapid Method for Isolation of 124 kDa Oat Phytochrome
A Simple and Rapid Method for Isolation of 124 kDa Oat Phytochrome
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一种简单快速的 124 kDa 燕麦光敏色素分离方法
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
W. Rüdiger
中科院分区：
文献类型：
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作者：
R. Grimm;W. Rüdiger
摘要以25%的产量从黄化燕麦幼苗中分离到原生（124 kDa）光敏色素。除若干离心步骤外，该程序仅包括一个色谱步骤，即羟基磷灰石柱。3 kg新鲜组织的大规模制备在16 h内完成。从SDS凝胶电泳和吸光度比A667 / A280 = 0.99推断，分离的光敏色素的纯度与以前的制备一样好，甚至更好
Abstract Native (124 kDa) phytochrome has been isolated from etiolated oat seedlings in 25% yield. The procedure includes only one chromatographic step namely a hydroxylapatite column, besides several centrifugation steps. Large scale preparations with 3 kg of fresh tissue are completed within 16 h. The purity of isolated phytochrome is as good as or even better than that of previous preparations as deduced from SDS gelelectrophoresis and from the absorbance ratio A667 / A280 = 0.99