Generation and characterization of monoclonal antibodies specific to Drosophila presenilin.
Generation and characterization of monoclonal antibodies specific to Drosophila presenilin.
复制标题
果蝇早老素特异性单克隆抗体的生成和表征。
DOI：
10.1089/hyb.2008.0086
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Koh,YoungHo
中科院分区：
文献类型：
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作者：
Cuc,HoThiThu;Seo,JongBok;Choi,JinKyu;Kim,WonTae;Park,SeokJou;Lee,DaeWeon;Kim,YongSun;Fortini,MarkE;Koh,YoungHo
The development of a monoclonal antibody (MAb) specific toDrosophila presenilin(Psn) proteinsin vivowas the major aim of this study, since the absence of specific antibodies recognizingPsnproteins hampered our progress in understandingPsnfunctions during development, differentiation, and pathogenesis. By dot blot and immunofluorescence screenings, we found that MAb Psn2G6 specifically recognizedPsnproteins in wing imaginal discs and brains of wild-type controlW1118larvae. MAb Psn2G6 also transgenically expressed a long form of wild-typePsn(Psn + 14 WT) proteins in wing imaginal discs of two independent transgenic lines. Transgenic expression ofPsn + 14 WTproteins inpsnB3larvae completely rescued the expression patterns ofPsnproteins and the development of wing imaginal discs. In addition, neural hyperplasia observed in wing imaginal discs ofpsnB3larvae was also suppressed.
