Expression pattern of sorting nexin 25 in temporal lobe epilepsy: A study on patients and pilocarpine-induced rats
Expression pattern of sorting nexin 25 in temporal lobe epilepsy: A study on patients and pilocarpine-induced rats
复制标题
颞叶癫痫中分选 nexin 25 的表达模式：患者和毛果芸香碱诱导的大鼠的研究
DOI：
10.1016/j.brainres.2013.03.005
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发表时间：
2013-05-06
期刊：
影响因子：
2.9
通讯作者：
Lue, Yang
中科院分区：
文献类型：
--
作者：
Du, Yingshi;Zou, Yan;Lue, Yang
目的：转化生长因子-β信号通路参与癫痫的发生。分选Nexin 25(SNX25)最近被提出通过对溶酶体降解的转化生长因子-β受体进行内体分选来调节转化生长因子-β信号转导。本研究的目的是确定SNX25在癫痫患者和癫痫动物模型中的表达模式。方法：采用Western blotting、实时定量RT-PCR和双标记免疫荧光技术检测SNX25在颞叶癫痫(TLE)和匹罗卡品致痫大鼠模型脑组织中的表达。结果：TLE组SNX25的表达显著高于对照组(0.21+/-0.07 vs.0.11+/-0.03，P
Purpose: The transforming growth factor beta (TGF-beta) signaling pathway is involved in the epileptogenesis. Sorting Nexin 25 (SNX25) has been recently proposed to modulate TGF-beta signaling through endosomal sorting of TGF-beta receptors for lysosomal degradation. The aim of the present study was to determine the expression pattern of SNX25 in brains of epilepsy patients and in animal model of epilepsy. Methods: We investigated the expression of SNX25 in the brain tissues of patients with temporal lobe epilepsy (TLE) and in the pilocarpine-induced rat model of epilepsy using western blotting, real-time quantitative RT-PCR, and double-label immunofluorescence. Results: The expression of SNX25 was significantly increased in TLE patients in comparison to controls (0.21 +/- 0.07 vs. 0.11 +/- 0.03, P