Vitamin B2 functionalized iron oxide nanozymes for mouth ulcer healing
Vitamin B2 functionalized iron oxide nanozymes for mouth ulcer healing
复制标题
维生素 B_2 功能化氧化铁纳米酶治疗口腔溃疡
DOI：
10.1007/s11427-019-9590-6
复制
发表时间：
2020-01-01
影响因子：
9.1
通讯作者：
Gao, Lizeng
中科院分区：
文献类型：
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作者：
Gu, Yunhao;Huang, Yixin;Gao, Lizeng
口腔溃疡与炎症和细菌感染的高风险有关，这使患者的病情恶化。目前，口腔溃疡没有有效的治疗方法。在此，我们报道了维生素修饰的氧化铁纳米颗粒通过抗炎和抗菌活性改善口腔溃疡的愈合。我们发现维生素B-2（VB 2）修饰的氧化铁纳米颗粒表现出增强的过氧化物酶样、过氧化氢酶样和超氧化物歧化酶（SOD）样活性，作为具有三联体活性的典型氧化铁纳米酶（IONzymes）。特别是，VB 2修饰显着提高了SOD样活性，从而提供了活性氧（ROS）清除能力。细胞抗氧化实验表明，维生素B-2修饰的离子酶（VB 2-IONzymes）对人口腔角质形成细胞（HOK）和BALB/3 T3细胞具有抗过氧化氢（H2 O2）损伤的保护作用，且与真核细胞具有良好的生物相容性。此外，VB 2-IONzymes对变形链球菌、金黄色葡萄球菌和大肠杆菌具有抗菌活性。重要的是，VB 2-IONzymes通过清除ROS和抗菌活性加速小鼠口腔溃疡模型的恢复并减少局部炎症因子的分泌。综上所述，我们的工作表明，维生素B-2修饰赋予氧化铁纳米颗粒增强的酶样活性，VB 2-离子酶可能是一个有前途的试剂，在口腔溃疡的治疗，因为它们固有的抗炎和抗菌能力。
Mouth ulcer is associated with inflammation and high risk of bacterial infection, which aggravates the patient's condition. Currently, there is no effective treatment for mouth ulcer. Herein, we report that vitamin-modified iron oxide nanoparticles improve the healing of mouth ulcer through anti-inflammation and antibacterial activities. We discovered that vitamin B-2 (VB2) modified iron oxide nanoparticles performed enhanced peroxidase-like, catalase-like, and superoxide dismutase (SOD)-like activities, acting as typical iron oxide nanozymes (IONzymes) with triad activities. In particular, VB2 modification significantly improved the SOD-like activity, thus providing a reactive oxygen species (ROS)-scavenging ability. Cellular antioxidant experiments showed that vitamin B-2 modified IONzymes (VB2-IONzymes) protect human oral keratinocytes (HOK) and BALB/3T3 cells from hydrogen peroxide (H2O2), and these cells have high biocompatibility to eukaryotic cells. In addition, VB2-IONzymes exerted an antibacterial activity against Streptococcus mutans, Staphylococcus aureus, and Escherichia coli. Importantly, VB2-IONzymes accelerated the recovery of mouth ulcer and reduced the local secretion of inflammatory factors in mouse ulcer model via ROS scavenging and antibacterial activity. Taken together, our work demonstrates that vitamin B-2 modification endows iron oxide nanoparticles with enhanced enzyme-like activities and VB2-IONzymes may be a promising reagent in the treatment of mouth ulcer because of their intrinsic anti-inflammation and antibacterial capabilities.