Diffusion of Knotted DNA Molecules in Nanochannels in the Extended de Gennes Regime
Diffusion of Knotted DNA Molecules in Nanochannels in the Extended de Gennes Regime
复制标题
DOI：
10.1021/acs.macromol.1c00143
复制
发表时间：
2021-04-20
期刊：
影响因子：
5.5
通讯作者：
Dorfman, Kevin D.
中科院分区：
文献类型：
--
作者：
Ma, Zixue;Dorfman, Kevin D.
我们研究了T4 GT 7脱氧核糖核酸（DNA）分子的扩散限制在纳米通道的有效尺寸为307 nm之前和之后结的形成。测得的DNA链扩散率为0.0243 +/- 0.0009 μ m（2）/s（对于未打结的DNA分子）和0.014 +/- 0.001 μ m（2）/s（对于含有结的DNA分子），估计结轮廓长度为9 +/- 2 μ m。在存在大的结的扩散率降低表明，DNA壁摩擦，而不是缩短的nondraining分子，占主导地位的摩擦打结的DNA在延长的德Gennes制度的纳米通道限制。
We study the diffusion of T4 GT7 deoxyribonucleic acid (DNA) molecules confined in nanochannels with an effective size of 307 nm before and after knot formation. The measured DNA chain diffusivity is 0.0243 +/- 0.0009 mu m(2)/s for unknotted DNA molecules and 0.014 +/- 0.001 mu m(2)/s for the DNA molecules that contain knots with an estimated knot contour length of 9 +/- 2 mu m. The reduced diffusivity in the presence of large knots indicates that the DNA-wall friction, rather than the shortening of the nondraining molecule, dominates the friction of knotted DNA in the extended de Gennes regime of nanochannel confinement.