Two-step-only phase-shifting interferometry with optimized detector bandwidth for microscopy of live cells
Two-step-only phase-shifting interferometry with optimized detector bandwidth for microscopy of live cells
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DOI：
10.1364/oe.17.015585
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发表时间：
2009-08-31
期刊：
影响因子：
3.8
通讯作者：
Wax, Adam
中科院分区：
文献类型：
--
作者：
Shaked, Natan T.;Zhu, Yizheng;Wax, Adam
我们提出了一种相移干涉技术，用于在生长介质中成像活生物细胞，同时优化空间分辨率并实现潜在的实时测量能力。该技术采用微离轴干涉，比传统的离轴干涉需要更少的探测器带宽，比传统的正轴干涉需要更少的测量量。并与传统的干涉测量方法进行了实验和理论比较。通过活的人皮肤癌细胞相显微复制实验证明了该方法的有效性。(C) 2009年美国光学学会
We present a phase-shifting interferometric technique for imaging live biological cells in growth media, while optimizing spatial resolution and enabling potential real-time measurement capabilities. The technique uses slightly-off-axis interferometry which requires less detector bandwidth than traditional off-axis interferometry and fewer measurements than traditional on-axis interferometry. Experimental and theoretical comparisons between the proposed method and these traditional interferometric approaches are given. The method is experimentally demonstrated via phase microcopy of live human skin cancer cells. (C) 2009 Optical Society of America