TEMPORAL AND TISSUE-SPECIFIC EXPRESSION OF MOUSE ETS GENES
TEMPORAL AND TISSUE-SPECIFIC EXPRESSION OF MOUSE ETS GENES
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DOI：
10.1073/pnas.84.10.3161
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发表时间：
1987-05-01
影响因子：
11.1
通讯作者：
PAPAS, TS
中科院分区：
文献类型：
--
作者：
BHAT, NK;FISHER, RJ;PAPAS, TS
ets基因的表达已经在小鼠组织和再生小鼠肝脏（一种细胞增殖的体内模型）中进行了研究。我们的结果表明：（i）ets-1和ets-2基因座是转录活性的;（ii）ets-2基因座编码一个主要的mRNA，（3.5-β-淀粉酶）并在大多数检查的组织中表达，而ets-1基因座编码主要的5.3-β-淀粉酶和次要的4.0-、2.5-和2.2-β-淀粉酶RNA种类，并在胸腺中以高水平表达;（iii）ets-1和ets-2 mRNA在年轻的增殖组织中是丰富的，并且在除胸腺之外的终末分化组织中是大大减少的;（iv）肝脏的补偿性生长在DNA合成之前诱导ets-2 mRNA，但是在fos和myc诱导之后;和（v）ets-2 mRNA，而不是ets-1 mRNA，在放线菌酮存在下在肝再生期间是稳定的。这些结果表明ets-2基因表达与细胞增殖有内在联系。因此，ets-2的表达遵循与核癌基因家族其他成员相似的模式。在肝再生过程中，ets-1和ets-2基因座受到不同的调节。
The expression of ets genes has been studied in mouse tissues and regenerating murine liver, an in vivo model for cell proliferation. Our results indicate that (i) the ets-1 and ets-2 loci are transcriptionally active; (ii) the ets-2 locus encodes a major mRNA (3.5 kilobases) and is expressed is most of the tissues examined, whereas the ets-1 locus encodes a major 5.3-kilobase and minor 4.0-, 2.5-, and 2.2-kilobase RNA species and is expressed at a high level in thymus; (iii) both ets-1 and ets-2 mRNA are abundant in young proliferating tissues and are greatly reduced in terminally differentiated tissues, except thymus; (iv) compensatory growth of liver induces ets-2 mRNA before DNA synthesis, but after fos and myc induction, and (v) ets-2 mRNA, but not ets-1 mRNA, is stabilized in the presence of cycloheximide during hepatic regeneration. These results suggest that ets-2 gene expression is intrinsically linked with cell proliferation. Thus, ets-2 expression follows a pattern similar to other members of the nuclear oncogene family. During hepatic regeneration, the ets-1 and ets-2 loci are subject to differential regulation.