Doublecortin expression levels in adult brain reflect neurogenesis
Doublecortin expression levels in adult brain reflect neurogenesis
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DOI：
10.1111/j.1460-9568.2004.03813.x
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发表时间：
2005-01-01
影响因子：
3.4
通讯作者：
Aigner, L
中科院分区：
文献类型：
--
作者：
Couillard-Despres, S;Winner, B;Aigner, L
神经发生领域的进展目前受到缺乏能够快速和定量分析成人大脑中神经发生的工具的限制。双皮质素（DCX）最近被用作神经发生的标志物。然而，尚不清楚DCX是否可用于评估成年哺乳动物中枢神经系统损伤或刺激因素后神经发生率的调节。使用两个范例增加神经发生水平（体力活动和癫痫发作），我们证明，量化的DCX表达细胞允许一个准确的测量调制成人神经发生率。重要的是，我们排除了在生理或反应性胶质细胞生成过程中诱导DCX表达，也排除了再生轴突生长过程中DCX再表达。我们的数据验证DCX作为一个可靠的和具体的标志物，反映了成人神经发生及其调制的水平。我们证明，DCX是一个有价值的替代技术，目前用于测量神经发生的水平。重要的是，与传统技术相比，通过检测DCX来分析神经发生不需要在体内标记增殖细胞，从而为在正常和病理条件下研究人类神经发生开辟了新的途径。
Progress in the field of neurogenesis is currently limited by the lack of tools enabling fast and quantitative analysis of neurogenesis in the adult brain. Doublecortin (DCX) has recently been used as a marker for neurogenesis. However, it was not clear whether DCX could be used to assess modulations occurring in the rate of neurogenesis in the adult mammalian central nervous system following lesioning or stimulatory factors. Using two paradigms increasing neurogenesis levels (physical activity and epileptic seizures), we demonstrate that quantification of DCX-expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis. Importantly, we excluded induction of DCX expression during physiological or reactive gliogenesis and excluded also DCX re-expression during regenerative axonal growth. Our data validate DCX as a reliable and specific marker that reflects levels of adult neurogenesis and its modulation. We demonstrate that DCX is a valuable alternative to techniques currently used to measure the levels of neurogenesis. Importantly, in contrast to conventional techniques, analysis of neurogenesis through the detection of DCX does not require in vivo labelling of proliferating cells, thereby opening new avenues for the study of human neurogenesis under normal and pathological conditions.