Comparison of the chemical and polypeptide composition of tubular and glomerular basement membranes.
Comparison of the chemical and polypeptide composition of tubular and glomerular basement membranes.
复制标题
肾小管和肾小球基底膜的化学和多肽组成的比较。
DOI：
10.1159/000172736
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Hudson,BG
中科院分区：
文献类型：
--
作者：
Butkowski,RJ;Grantham,JJ;Hudson,BG
从兔、大鼠和小鼠肾脏分离肾小管和肾小球基底膜。开发了从有限数量的动物中获得基底膜的方法。兔和小鼠肾小管基底膜以及兔和大鼠肾小球基底膜从单个动物中获得，数量足以进行电泳和化学分析。比较了所有基底膜的化学组成，并对兔肾小管和肾小球基底膜进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，以比较它们的多肽组成。肾小管基底膜的化学组成相似，每个物种分别具有几乎相等浓度的甘氨酸、羟脯氨酸和羟赖氨酸。肾小球基底膜的化学组成也相似，与肾小管基底膜接近。在每个物种中，肾小管基底膜的甘氨酸、羟脯氨酸和羟赖氨酸值比肾小球基底膜高约10%。减少，十二烷基硫酸钠可溶性级分的兔基底膜的多肽组合物似乎是相似的，有两个例外。在肾小管基底膜凝胶中观察到的Mr = 160，000的显著带在肾小球基底膜样品中作为淡染色带存在，在肾小球基底膜凝胶中观察到的Mr = 140，000的显著带在肾小管基底膜样品中作为淡染色带存在。
Renal tubular and glomerular basement membranes were isolated from rabbit, rat and mouse kidneys. Methods were developed to obtain the basement membrane from limited numbers of animals. Rabbit and mouse tubular basement membrane as well as rabbit and rat glomerular basement membrane were obtained from single animals in quantities sufficient for electrophoresis and chemical analysis. The chemical composition of all the basement membranes were compared and sodium dodecylsulfate-polyacrylamide gel electrophoresis was performed on rabbit tubular and glomerular basement membranes in order to compare their polypeptide composition. The chemical composition of the tubular basement membranes were similar, each species having nearly equal concentrations of glycine, hydroxyproline and hydroxylysine, respectively. Glomerular basement membranes were also similar to each other and close to tubular basement membranes in chemical composition. Within each species, the glycine, hydroxyproline and hydroxylysine values for tubular basement membrane were about 10% higher than for glomerular basement membrane. The polypeptide composition of a reduced, sodium dodecylsulfate-soluble fraction of the rabbit basement membranes appeared to be alike with two exceptions. A prominent band of Mr = 160,000 seen in gels of tubular basement membrane was present as a lightly staining band in glomerular basement membrane samples and a prominent band of Mr = 140,000 in gels of glomerular basement membrane was seen as a light band in samples of tubular basement membrane.