Lysophosphatidylserine stimulates chemotactic migration of colorectal cancer cells through GPR34 and PI3K/Akt pathway.
Lysophosphatidylserine stimulates chemotactic migration of colorectal cancer cells through GPR34 and PI3K/Akt pathway.
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发表时间：
2014-10
影响因子：
2
通讯作者：
Yuuki Iida;Nelson H Tsuno;J. Kishikawa;Kensuke Kaneko;K. Murono;K. Kawai;T. Ikeda;S. Ishihara;H. Yamaguchi;E. Sunami;J. Kitayama;Y. Yatomi;Toshiaki Watanabe
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作者：
Yuuki Iida;Nelson H Tsuno;J. Kishikawa;Kensuke Kaneko;K. Murono;K. Kawai;T. Ikeda;S. Ishihara;H. Yamaguchi;E. Sunami;J. Kitayama;Y. Yatomi;Toshiaki Watanabe
背景溶血磷脂酰丝氨酸(LlysoPS)是一种溶血磷脂介质，与变态反应和肿瘤进展密切相关。我们研究了溶菌素对结直肠癌(CRC)细胞的生理功能，以及相关的受体和信号通路。材料与方法采用逆转录-聚合酶链式反应(RT-PCR)检测6种细胞株上溶菌素受体的表达。对溶菌素的生理功能进行了研究，并用小干扰RNA(SiRNA)或信号通路的抑制剂进行了实验。结果在3种溶菌素受体中，GPR34在所有细胞株上均有高表达。LysoPS刺激趋化迁移能力。Wortmannin可抑制PI3K/Akt细胞的迁移能力和GPR34的表达，提示该受体参与了PI3K/Akt信号通路。结论本研究证实了溶菌素诱导细胞迁移的受体和途径，为靶向防治结直肠癌的新策略奠定了基础。
BACKGROUND Lysophosphatidylserine (lysoPS) is a type of lysophospholipid mediator, which is involved in allergic conditions and tumor progression. We investigated the physiological function of lysoPS on colorectal cancer (CRC) cell lines, as well as the involved receptor and signaling pathways. MATERIALS AND METHODS Expression of lysoPS receptors on six cell lines was examined by reverse transcription-polymerase chain reaction (RT-PCR). The physiological functions of lysoPS were investigated, and experiments using small interfering RNA (siRNA) or inhibitors of the signaling pathways were conducted. RESULTS Among the three lysoPS receptors, GPR34 was highly expressed on all cell lines. LysoPS stimulated the chemotactic migratory ability. Wortmannin inhibited the migratory ability, as well as the GPR34 knock-down, strongly suggestive of the involvement of this receptor in the PI3K/Akt pathway. CONCLUSION The involved receptor and pathways in the migratory ability in response to lysoPS was demonstrated, which opens premises for targeting as a new strategy for prevention and treatment of colorectal cancer.