Surface modification of MPEG-b-PCL-based nanoparticles via oxidative self-polymerization of dopamine for malignant melanoma therapy.
Surface modification of MPEG-b-PCL-based nanoparticles via oxidative self-polymerization of dopamine for malignant melanoma therapy.
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DOI：
10.2147/ijn.s79605
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发表时间：
2015
影响因子：
8
通讯作者：
Li Q
中科院分区：
文献类型：
--
作者：
Xiong W;Peng L;Chen H;Li Q
为提高化疗药物对恶性黑色素瘤的治疗效果，制备了紫杉醇（PTX）-聚乙二醇-b-聚己内酯纳米粒（MPEG-b-PCL NPs@PDA）。采用开环聚合法合成了嵌段共聚物MPEG-b-PCL，并通过核磁共振波谱和凝胶渗透色谱对其进行了表征。通过改进的纳米沉淀技术制备载PTX的纳米颗粒。在尺寸和尺寸分布、zeta电位、表面形态、药物包封效率和药物释放方面表征PTX负载的NPs和PTX负载的NPs@PDA。激光共聚焦扫描显微镜显示，香豆素-6-纳米粒子可被人黑色素瘤细胞系A875内化。经PDA修饰后，细胞对纳米粒的摄取效率大大提高。在体外通过3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四唑（MTT）测定和在体内通过异种移植肿瘤模型研究了PTX负载的NPs@PDA的抗肿瘤功效。与Taxol®和负载紫杉醇的前体NP相比，负载紫杉醇的NP @PDA可以显着抑制肿瘤生长。所有结果表明，PTX负载的MPEG-b-PCL纳米粒子，其表面修饰的PDA是有希望的恶性黑色素瘤治疗的纳米载体。
To enhance the therapeutic effects of chemotherapy on malignant melanoma, paclitaxel (PTX)-loaded methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) nanoparticles (MPEG-b-PCL NPs) that had their surfaces modified with polydopamine (PTX-loaded MPEG-b-PCL NPs@PDA) were prepared as drug vehicles. The block copolymer MPEG-b-PCL was synthesized by ring-opening polymerization and characterized by proton nuclear magnetic resonance spectroscopy and gel permeation chromatography. The PTX-loaded NPs were prepared by a modified nanoprecipitation technique. The PTX-loaded NPs and PTX-loaded NPs@PDA were characterized in terms of size and size distribution, zeta potential, surface morphology, drug encapsulation efficiency, and drug release. Confocal laser scanning microscopy showed that coumarin-6-loaded NPs@PDA could be internalized by human melanoma cell line A875 cells. The cellular uptake efficiency of NPs was greatly enhanced after PDA modification. The antitumor efficacy of the PTX-loaded NPs@PDA was investigated in vitro by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and in vivo by a xenograft tumor model. The PTX-loaded NPs@PDA could significantly inhibit tumor growth compared to Taxol® and precursor PTX-loaded NPs. All the results suggested that the PTX-loaded MPEG-b-PCL NPs that had their surfaces modified with PDA are promising nanocarriers for malignant melanoma therapy.