Porphyromonas gingivalis promotes murine abdominal aortic aneurysms via matrix metalloproteinase-2 induction
Porphyromonas gingivalis promotes murine abdominal aortic aneurysms via matrix metalloproteinase-2 induction
复制标题
牙龈卟啉单胞菌通过基质金属蛋白酶-2诱导促进小鼠腹主动脉瘤
DOI：
10.1111/j.1600-0765.2010.01326.x
复制
发表时间：
2011-04-01
影响因子：
3.5
通讯作者：
Isobe, M.
中科院分区：
文献类型：
--
作者：
Aoyama, N.;Suzuki, J.;Isobe, M.
背景与目的：腹主动脉瘤（AAA）是一种常见的致死性疾病，基质金属蛋白酶（MMPs）在AAA病变中高表达。大量的牙周病细菌已被报道存在于从AAA患者的主动脉壁获得的标本中。本研究的目的是分析牙周致病菌对AAA dilatation.Material和方法的影响：AAAs产生的腹主动脉周围应用0.25 m氯化钙，和NaCl作为对照。结果：在主动脉周围应用CaCl 2或NaCl后4周，与载体对照组小鼠相比，牙龈卟啉单胞菌攻击组小鼠的主动脉直径显著增加（p < 0.05），而A.放线菌共生菌攻击的小鼠。免疫组织化学分析发现，与对照组小鼠相比，牙龈卟啉单胞菌攻击小鼠的口腔黏膜样本中CD 8阳性和MOMA 2阳性细胞的数量显著增加，MMP-2的水平显著升高。与牙龈卟啉单胞菌-脂多糖和活A.结论：牙龈卟啉单胞菌对放线菌伴放线菌感染有明显的抑制作用，而放线菌伴放线菌感染对牙龈卟啉单胞菌感染无明显抑制作用。放线菌共生菌，可以加速，甚至启动，通过增加MMP的表达实验性AAA的进展。
Background and Objective:Abdominal aortic aneurysm (AAA) is a common and lethal disorder, and MMPs are highly expressed in AAA lesions. Large numbers of periodontopathic bacteria have been reported to be present in specimens obtained from the aortic walls of patients with an AAA. The purpose of this study was to analyze the influence of periodontopathic bacteria on AAA dilatation.Material and Methods:AAAs were produced in mice by the periaortic application of 0.25 m CaCl2, and NaCl was used as a control. The mice were inoculated once weekly with live Porphyromonas gingivalis, live Aggregatibacter actinomycetemcomitans or vehicle.Results:Four weeks after the periaortic application of either CaCl2 or NaCl, a significant increase was observed in the aortic diameter of P. gingivalis-challenged mice compared with the vehicle control mice (p < 0.05), whereas there was no statistically significant increase in the aortic diameter of the A. actinomycetemcomitans-challenged mice. Immunohistochemical analysis found significantly higher numbers of CD8-positive and MOMA2-positive cells and significantly higher levels of MMP-2 in the aneurysmal samples of P. gingivalis-challenged mice compared with control mice. Live P. gingivalis promoted a significant proliferation of splenocytes in comparison with P. gingivalis-lipopolysaccharide and live A. actinomycetemcomitans (p < 0.05).Conclusion:These findings demonstrate that challenge with P. gingivalis, but not with A. actinomycetemcomitans, can accelerate, or even initiate, the progression of experimental AAA through the increased expression of MMPs.