Cortactin associates with N-cadherin adhesions and mediates intercellular adhesion strengthening in fibroblasts
Cortactin associates with N-cadherin adhesions and mediates intercellular adhesion strengthening in fibroblasts
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DOI：
10.1242/jcs.01385
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发表时间：
2004-10-01
影响因子：
4
通讯作者：
McCulloch, CAG
中科院分区：
文献类型：
--
作者：
El Sayegh, TY;Arora, PD;McCulloch, CAG
成纤维细胞中N-钙粘蛋白介导的细胞间粘附强度的调节特征很差;这部分是由于缺乏可用的定量模型。我们使用重组N-钙粘蛋白嵌合蛋白和大鼠2成纤维细胞，供体-受体细胞模型，研究皮层肌动蛋白丝和corneum在加强N-钙粘蛋白粘附的重要性。在洗脱试验中，细胞松弛素D（1 μ M）使细胞间粘附减少了三倍，证实了皮质肌动蛋白丝在加强N-钙粘蛋白介导的粘附中的重要性。Cordyn是一种肌动蛋白丝结合蛋白，空间共定位于新生的N-钙粘蛋白粘附复合物，并与之直接相关。与对照组相比，用皮质素特异性RNAi寡核苷酸转染Rat-2细胞，皮质素蛋白减少85%，细胞间粘附减少两倍（P
The regulation of N-cadherin-mediated intercellular adhesion strength in fibroblasts is poorly characterized; this is due, in part, to a lack of available quantitative models. We used a recombinant N-cadherin chimeric protein and a Rat 2 fibroblast, donor-acceptor cell model, to study the importance of cortical actin filaments and cortactin in the strengthening of N-cadherin adhesions. In wash-off assays, cytochalasin D (1 muM) reduced intercellular adhesion by threefold, confirming the importance of cortical actin filaments in strengthening of N-cadherin-mediated adhesions. Cortactin, an actin filament binding protein, spatially colocalized to, and directly associated with, nascent N-cadherin adhesion complexes. Transfection of Rat-2 cells with cortactinspecific, RNAi oligonucleotides reduced cortactin protein by 85% and intercellular adhesion by twofold compared with controls (P