Doublestranded DNA of soybean chlorotic mottle virus
Doublestranded DNA of soybean chlorotic mottle virus
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DOI：
10.3186/jjphytopath.52.785
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发表时间：
1986-12
影响因子：
2.6
通讯作者：
T. Hibi;M. Iwaki;Yasuo Saito;J. Verver;R. Goldbach
中科院分区：
文献类型：
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作者：
T. Hibi;M. Iwaki;Yasuo Saito;J. Verver;R. Goldbach
对大豆褪绿斑驳病毒（Soybean chlorotic mottle virus，CMV）的核酸进行了分离和分析。CsCl平衡密度梯度离心和热变性动力学表明，核酸是一个双链DNA。琼脂糖凝胶电泳将黄瓜花叶病毒DNA分离成多条带，与花椰菜花叶病毒（CaMV）DNA相似，表明该DNA由线性和环状分子组成。这些不同的拓扑形式的存在，证实了电子显微镜。用六种不同的限制性内切酶构建了一个物理图谱，该图谱显示CMV基因组是一个大约8，200个碱基对长的环状DNA分子。所获得的结果支持先前的分配的CMV的花椰菜花叶病毒组。
The nucleic acid of soybean chlorotic mottle virus (SoyCMV) was isolated and analysed. CsCl equilibrium density gradient centrifugation and thermal denaturation kinetics revealed that the nucleic acid is a double-stranded DNA. Electrophoresis in agarose gels separated SoyCMV DNA into multiple bands, similar to those of cauliflower mosaic virus (CaMV) DNA, which indicated that this DNA consisted of both linear and circular molecules. The existence of these different topological forms was confirmed by electron microscopy. Using six different restriction endonucleases a physical map was constructed which showed the SoyCMV genome to be a circular DNA molecule of approximately 8, 200 base pairs long. The results obtained support the previous assignment of SoyCMV to the caulimovirus group.