The combined effect of encapsulating curcumin and C6 ceramide in liposomal nanoparticles against osteosarcoma.
The combined effect of encapsulating curcumin and C6 ceramide in liposomal nanoparticles against osteosarcoma.
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DOI：
10.1021/mp400366r
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发表时间：
2014-02-03
影响因子：
4.9
通讯作者：
Pochampally R
中科院分区：
文献类型：
--
作者：
Dhule SS;Penfornis P;He J;Harris MR;Terry T;John V;Pochampally R
本研究探讨了姜黄素和C6神经酰胺（C6）对骨肉瘤（OS）细胞系的抗肿瘤潜力时，都封装在双层脂质体纳米粒子。制备三种脂质体制剂-姜黄素脂质体、C6脂质体和C6-姜黄素脂质体。与单独的姜黄素脂质体相比，姜黄素与C6组合在MG-63和KHOS OS细胞系的情况下显示出1.5倍增强的细胞毒性作用。重要的是，发现C6-姜黄素脂质体与OS细胞系相比对未转化的人细胞（人间充质干细胞）的毒性较小。此外，对KHOS细胞系的细胞周期测定显示，姜黄素脂质体通过上调细胞周期蛋白B1诱导G2/M期阻滞，而C6脂质体通过下调细胞周期蛋白D1诱导G1期阻滞。C6-姜黄素脂质体诱导G2/M期阻滞，并表现出细胞周期蛋白D1和细胞周期蛋白B1的表达水平的联合作用。使用人骨肉瘤异种移植物测定在体内测试制剂的效率。使用聚乙二醇化脂质体来增加血浆半衰期并用叶酸（FA）标记用于体内靶向递送，用C6-姜黄素-FA脂质体观察到肿瘤大小的显著减小。两种水不溶性药物姜黄素和C6在脂质体的脂质双层中的包封增强了细胞毒性作用，并验证了联合药物治疗的潜力。
This study examines the anti-tumor potential of curcumin and C6 ceramide (C6) against osteosarcoma (OS) cell lines when both are encapsulated in the bilayer of liposomal nanoparticles. Three liposomal formulations were prepared – curcumin liposomes, C6 liposomes and C6-curcumin liposomes. Curcumin in combination with C6 showed 1.5 times enhanced cytotoxic effect in the case of MG-63 and KHOS OS cell lines, in comparison with curcumin liposomes alone. Importantly, C6-curcumin liposomes were found to be less toxic on untransformed human cells (human mesenchymal stem cells) in comparison to OS cell lines. In addition, cell cycle assays on a KHOS cell line after treatment revealed that curcumin only liposomes induced G2/M arrest by upregulation of cyclin B1, while C6 only liposomes induced G1 arrest by downregulation of cyclin D1. C6-curcumin liposomes induced G2/M arrest and showed a combined effect in the expression levels of cyclin D1 and cyclin B1. The efficiency of the preparations was tested in vivo using a human osteosarcoma xenograft assays. Using pegylated liposomes to increase the plasma half-life and tagging with folate (FA) for targeted delivery in vivo, a significant reduction in tumor size was observed with C6-curcumin-FA liposomes. The encapsulation of two water insoluble drugs, curcumin and C6, in the lipid bilayer of liposomes enhances the cytotoxic effect and validates the potential of combined drug therapy.
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在连续和脉冲给药策略期间对靶向抗癌疗法的抵抗力的演变。
影响因子：
4.3
作者：
Foo J;Michor F
通讯作者：
Michor F
影响因子：
3.4
作者：
Lopez-Montero, Ivan;Velez, Marisela;Devaux, Philippe F.
通讯作者：
Devaux, Philippe F.
影响因子：
2.9
作者：
Miller, CR;Bondurant, B;O'Brien, DF
通讯作者：
O'Brien, DF
DOI：
10.1073/pnas.84.13.4490
发表时间：
1987-07-01
影响因子：
11.1
作者：
KUCZEK, T;AXELROD, DE
通讯作者：
AXELROD, DE
影响因子：
5.8
作者：
Chearwae, W;Anuchapreeda, S;Limtrakul, P
通讯作者：
Limtrakul, P