Identification of Mendel's White Flower Character
Identification of Mendel's White Flower Character
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DOI：
10.1371/journal.pone.0013230
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发表时间：
2010-10-11
期刊：
影响因子：
3.7
通讯作者：
Ellis, T. H. Noel
中科院分区：
文献类型：
--
作者：
Hellens, Roger P.;Moreau, Carol;Ellis, T. H. Noel
背景：花色的遗传调控已被广泛研究，特别是作为孟德尔等人在豌豆遗传研究中使用的一个性状。方法/主要发现：我们利用模式豆科植物的基因组序列，以及它们与豌豆基因组的已知同属性来鉴定豌豆中A和A2位点的候选基因。然后，我们使用遗传定位、快中子突变分析、等位基因多样性、转录物定量和瞬时表达互补研究相结合的方法来确认候选基因的身份。结论/意义：我们鉴定出了豌豆基因A和A2。A是决定豌豆花青素色素沉着的因素，150年前孟德尔在他的遗传研究中使用了A。A基因编码bHLH转录因子。孟德尔最有可能使用的白花突变等位基因是剪接供体位点的简单G到a过渡，导致带有过早终止密码子的错误剪接mRNA，我们已经确定了第二个罕见的突变等位基因。A2基因编码WD40蛋白，该蛋白是进化上保守的调节复合体的一部分。
Background: The genetic regulation of flower color has been widely studied, notably as a character used by Mendel and his predecessors in the study of inheritance in pea.Methodology/Principal Findings: We used the genome sequence of model legumes, together with their known synteny to the pea genome to identify candidate genes for the A and A2 loci in pea. We then used a combination of genetic mapping, fast neutron mutant analysis, allelic diversity, transcript quantification and transient expression complementation studies to confirm the identity of the candidates.Conclusions/Significance: We have identified the pea genes A and A2. A is the factor determining anthocyanin pigmentation in pea that was used by Gregor Mendel 150 years ago in his study of inheritance. The A gene encodes a bHLH transcription factor. The white flowered mutant allele most likely used by Mendel is a simple G to A transition in a splice donor site that leads to a mis-spliced mRNA with a premature stop codon, and we have identified a second rare mutant allele. The A2 gene encodes a WD40 protein that is part of an evolutionarily conserved regulatory complex.