Neurogenesis and angiogenesis within the ipsilateral thalamus with secondary damage after focal cortical infarction in hypertensive rats
Neurogenesis and angiogenesis within the ipsilateral thalamus with secondary damage after focal cortical infarction in hypertensive rats
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DOI：
10.1038/jcbfm.2009.76
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发表时间：
2009-09-01
影响因子：
6.3
通讯作者：
Zhang, Suping
中科院分区：
文献类型：
--
作者：
Ling, Li;Zeng, Jinsheng;Zhang, Suping
脑室下区和梗塞周围区域的神经发生和血管发生已得到证实。然而，中风后出现继发性损伤的丘脑非缺血同侧腹后核（VPN）中出现的新形成的神经元细胞和血管尚未被研究过。对24只易发生脑卒中的肾血管性高血压大鼠进行远端右大脑中动脉闭塞（MCAO）或假手术。 5'-Bromo-2'-脱氧尿苷 (BrdU) 用于标记细胞增殖。术后7或14天处死大鼠。在同侧VPN内检测到神经元核(NeuN)、OX-42、BrdU、巢蛋白、层粘连蛋白(+)、BrdU(+)/巢蛋白(+)、BrdU(+)/NeuN(+)、巢蛋白(+)/GFAP(+)(胶质纤维酸性蛋白)和BrdU(+)/层粘连蛋白(+)免疫反应细胞。原发性梗塞局限于右侧体感皮层。缺血大鼠同侧VPN内，NeuN(+)神经元数量减少，OX-42(+)小胶质细胞被激活，MCAO后第7天检测到BrdU(+)和nestin(+)细胞，第14天检测到BrdU(+)/nestin(+)细胞和BrdU(+)/NeuN(+)细胞，第14天检测到BrdU(+)/nestin(+)细胞和BrdU(+)/NeuN(+)细胞。此外，与假手术大鼠相比，缺血大鼠同侧VPN的血管密度显着增加。这些结果表明，局灶性皮质梗塞后，丘脑同侧 VPN 的神经发生和血管发生造成继发性损伤。脑血流与代谢杂志 (2009) 29, 1538-1546; doi：10.1038/jcbfm.2009.76； 2009 年 6 月 17 日在线发布
Neurogenesis and angiogenesis in the subventricular zone and peri-infarct region have been confirmed. However, newly formed neuronal cells and blood vessels that appear in the nonischemic ipsilateral ventroposterior nucleus (VPN) of the thalamus with secondary damage after stroke has not been previously studied. Twenty-four stroke-prone renovascular hypertensive rats were subjected to distal right middle cerebral artery occlusion (MCAO) or sham operation. 5'-Bromo-2'-deoxyuridine (BrdU) was used to label cell proliferation. Rats were killed at 7 or 14 days after the operation. Neuronal nuclei (NeuN), OX-42, BrdU, nestin, laminin(+), BrdU(+)/nestin(+), BrdU(+)/NeuN(+), nestin(+)/GFAP(+) (glial fibrillary acidic protein), and BrdU(+)/laminin(+) immunoreactive cells were detected within the ipsilateral VPN. The primary infarction was confined to the right somatosensory cortex. Within the ipsilateral VPN of the ischemic rats, the number of NeuN(+) neurons decreased, the OX-42(+) microglia cells were activated, and BrdU(+) and nestin(+) cells were detected at day 7 after MCAO and increased in number at day 14. Moreover, BrdU(+)/nestin(+) cells and BrdU(+)/NeuN(+) cells were detected at day 14 after MCAO. In addition, the ischemic rats showed a significant increase in vascular density in the ipsilateral VPN compared with the sham- operated rats. These results suggest that secondary damage with neurogenesis and angiogenesis of the ipsilateral VPN of the thalamus occurs after focal cortical infarction. Journal of Cerebral Blood Flow & Metabolism (2009) 29, 1538-1546; doi:10.1038/jcbfm.2009.76; published online 17 June 2009