Algicidal Activity of Marine Alteromonas sp KNS-16 and Isolation of Active Compounds
Algicidal Activity of Marine Alteromonas sp KNS-16 and Isolation of Active Compounds
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DOI：
10.1271/bbb.120102
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发表时间：
2012-08-01
影响因子：
1.6
通讯作者：
Cho, Ji Young
中科院分区：
文献类型：
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作者：
Cho, Ji Young
KNS-16溶藻菌是从赤潮发生区分离到的一株溶藻菌，经16 SrDNA序列测定，确定为交替单胞菌属（Alteromonas sp.）。发现KNS-16菌株通过在间接相互作用中产生杀藻化合物来控制赤潮。从KNS-16培养物中分离得到四个活性化合物，并通过核磁共振和质谱数据分析其结构。经结构鉴定为2-十一烯-1 '-基-4-喹诺酮（1）、2-十一烷基-4-喹诺酮（2）、3-己基-6-戊基-4-羟基-2H-吡喃-2-酮（3）和6-庚基-3-己基-4-羟基-2H-吡喃-2-酮（4）。化合物1对赤潮异弯藻（Heterosigma akashiwo）、聚克里藻（Coalodinium polykrikoides）和塔玛亚历山大藻（Alexandrum tamarense）等赤潮生物最具活性，LC 50值为0.5-1.1 μ g/mL。这四种化合物对水生藻类如Tetaselmis suecica、Isochrysis galbana和Pavlova lutheri的LC 50值为39-66 μ g/mL。基于对卤虫和轮虫的毒性测试，四种化合物对这两种生物的LC 50分别为409-608和189-224 μ g/mL。对许氏黄姑鱼幼鱼的LC 50值为284-304 μ g/mL。
The KNS-16 algicidal strain was isolated from a harmful alga bloom (HAB) area and identified as Alteromonas sp. based on 16S rDNA sequencing. The KNS-16 strain was found to control HABs by producing algicidal compounds in an indirect interaction. Four active compounds were isolated from KNS-16 culture, and their structures were analyzed by interpreting nuclear magnetic resonance and mass spectroscopy data. The structures were identified as 2-undecen-1'-yl-4-quinolone (1), 2-undecyl-4-quinolone (2), 3-hexyl-6-pentyl-4-hydroxyl-2H-pyran-2-one (3), and 6-heptyl-3-hexyl-4-hydroxyl-2H-pyran-2-one (4). Compound 1 was most active against HABs such as Heterosigma akashiwo, Cochlodinium polykrikoides, and Alexandrium tamarense with LC50 values of 0.5-1.1 mu g/mL. The four compounds exhibited high LC50 values against aqua-culture algae such as Tetaselmis suecica, Isochrysis galbana, and Pavlova lutheri at 39-66 mu g/mL. Based on toxicity tests on the brine shrimp Artemia sauna and the rotifer Brachionus rotundiformis, the four compounds showed ranges of 409-608 and 189-224 mu g/mL of LC50 for the two organisms, respectively. The LC50 values for juvenile fish of Sebastes schlegelii were 284-304 mu g/mL.