A Simple, Reliable, and Fast Protocol for Thraustochytrid DNA Extraction
A Simple, Reliable, and Fast Protocol for Thraustochytrid DNA Extraction
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简单、可靠、快速的破囊壶菌 DNA 提取方案
DOI：
10.1007/s101260000069
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发表时间：
2001
影响因子：
3
通讯作者：
B. Rinkevich
中科院分区：
文献类型：
--
作者：
C. Mo;B. Rinkevich
破囊壶菌是常见的海洋单细胞生物，其DNA提取通常采用十六烷基三甲基溴化铵（CTAB）或蛋白酶K法。描述了一种用于破囊壶菌总DNA提取的新型裂解缓冲液方案。平均分离的总DNA为20至40 kb，DNA样品适用于各种用途，包括18 S-核糖体DNA聚合酶链反应，限制性内切酶和扩增片段长度多态性分析。新协议也比其他协议更快。
DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol for thraustochytrid total DNA extraction is described. The average isolated total DNA is 20 to 40 kb, and DNA samples are suitable for a variety of uses including 18S–ribosomal DNA polymerase chain reaction, restriction enzyme digestions, and amplified fragment length polymorphism analyses. The new protocol is also faster than the other protocols.