Comparative study of kinetics on DNA double-strand break induced by photo- and gamma-irradiation: Protective effect of water-soluble flavonoids
Comparative study of kinetics on DNA double-strand break induced by photo- and gamma-irradiation: Protective effect of water-soluble flavonoids
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DOI：
10.1016/j.cplett.2010.11.003
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发表时间：
2010-12-06
影响因子：
2.8
通讯作者：
Yoshikawa, Kenichi
中科院分区：
文献类型：
--
作者：
Yoshikawa, Yuko;Mori, Toshiaki;Yoshikawa, Kenichi
用荧光显微镜对可见光和C射线诱导的DNA双链断裂进行了单分子观察。我们以定量的方式评估了黄酮类化合物的水溶性衍生物，α-葡糖基橙皮苷（α G-HSP）和α-葡糖基芦丁（α G-rutin）的保护作用。关于光诱导的损伤，发现α-G-芸香苷提供的保护是α-G-HSP的两倍，而α-G-芸香苷对c-射线照射的保护作用与α-G-HSP相当。这一观察结果表明这些化合物的抗氧化潜力依赖于辐射源。(C)2010 Elsevier B. V.保留所有权利。
The double-strand break of DNA induced by visible light and c-rays was studied through a single-molecule observation using fluorescence microscopy. We evaluated the protective effects of water-soluble derivatives of flavonoids, alpha-glucosyl hesperidin (alpha G-HSP) and alpha-glucosyl rutin (alpha G-rutin) in a quantitative manner. With regard to photo-induced damage, alpha G-rutin was found to provide twice as much protection as alpha G-HSP, whereas the protective effect of alpha G-rutin against c-ray irradiation was comparable with that of alpha G-HSP. This observation demonstrates the dependence of the antioxidant potentials of these compounds on the source of irradiation. (C) 2010 Elsevier B.V. All rights reserved.