Humoral immune response to conserved epitopes of Chlamydia trachomatis and human 60-kDa heat-shock protein in women with pelvic inflammatory disease
Humoral immune response to conserved epitopes of Chlamydia trachomatis and human 60-kDa heat-shock protein in women with pelvic inflammatory disease
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DOI：
10.1086/514218
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发表时间：
1998-03-01
影响因子：
6.4
通讯作者：
Witkin, SS
中科院分区：
文献类型：
--
作者：
Domeika, M;Domeika, K;Witkin, SS
对129例经腹腔镜证实的盆腔炎患者进行了沙眼衣原体60 kDa热休克蛋白（hsp 60）特异性和保守性抗原表位的体液免疫与人hsp 60免疫的相关性研究。用ELISA法检测了抗衣原体IgG和伊加抗体、抗重组人hsp 60 IgG抗体和抗衣原体hsp 60两种合成肽抗体。半数患者有抗人hsp 60抗体，这与衣原体hsp 60肽260-271抗体的存在相关，肽260-271与人hsp 60同源（P = 0.01）。肽260-271抗体与抗衣原体IgE（P <0.0001）和伊加（P <0.0001）相关。结果提示，C.女性沙眼衣原体上生殖道感染，可能是对衣原体hsp 60表位的免疫应答与人hsp 60交叉反应的结果。
The association between humoral immunity to unique and conserved epitopes of the Chlamydia trachomatis 60-kDa heat-shock protein (hsp60) and immunity to human hsp60 was examined in 129 women with laparoscopically verified pelvic inflammatory disease, An ELISA was used to detect antichlamydial IgG and IgA antibodies, IgG antibodies to recombinant human hsp60, and antibodies to two synthetic peptides of chlamydial hsp60. Half of the patients had antibodies to human hsp60, which correlated with the presence of antibodies to the chlamydial hsp60 peptide 260-271 homologous to the human hsp60 (P = .01), Antibodies to peptide 260-271 were associated with antichlamydial Ige (P < .0001) and IgA (P < .0001). The results suggest that the autoimmune response to human hsp60 can develop following C. trachomatis upper genital tract infection in women, probably as a consequence of an immune response to an epitope of chlamydial hsp60 crossreactive with the human hsp60.