Parallel synthesis and screening of a solid phase carbohydrate library
Parallel synthesis and screening of a solid phase carbohydrate library
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DOI：
10.1126/science.274.5292.1520
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发表时间：
1996-11-29
期刊：
影响因子：
56.9
通讯作者：
Kahne, D
中科院分区：
文献类型：
--
作者：
Liang, R;Yan, L;Kahne, D
合成了一个固相碳水化合物文库，并对其进行了抗紫荆花凝集素筛选。该文库含有约1300个二糖和三糖，是通过在TentaGel树脂上进行化学编码而合成的，每个珠子都含有一个碳水化合物。已经确定了两个比Gal-β-1，3-GalNAc(已知的配体)更紧密地与凝集素结合的配体。概述的策略可用于识别任何受体的碳水化合物配体；然而，由于衍生珠模拟细胞表面碳水化合物的多价呈现，筛选可能被证明对于发现与参与细胞黏附的蛋白质结合的新化合物特别有价值。
A solid phase carbohydrate library was synthesized and screened against Bauhinia purpurea lectin. The library, which contains approximately 1300 di- and trisaccharides, was synthesized with chemical encoding on TentaGel resin so that each bead contained a single carbohydrate. Two ligands that bind more tightly to the lectin than Gal-beta-1,3-GalNAc (the known ligand) have been identified. The strategy outlined can be used to identify carbohydrate-based ligands for any receptor; however, because the derivatized beads mimic the polyvalent presentation of cell surface carbohydrates, the screen may prove especially valuable for discovering new compounds that bind to proteins participating in cell adhesion.