Pigment cells trigger the onset of gastrulation in tropical sea urchin Echinometra mathaei
Pigment cells trigger the onset of gastrulation in tropical sea urchin Echinometra mathaei
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色素细胞触发热带海胆 Echinometra mathaei 的原肠胚形成
DOI:
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发表时间:
2004
期刊:
影响因子:
--
通讯作者:
T. Kominami
中科院分区:
文献类型:
--
作者:
H. Takata;T. Kominami
In the tropical sea urchin Echinometra mathaei, pigment cells are just detectable before the onset of gastrulation, owing to an early accumulation of red pigment granules. Taking advantage of this feature, behavior of pigment cells was studied in relation to the processes of gastrulation. Before the initiation of primary invagination, pigment cells were arranged in a hemi‐circle in the dorsal half of the vegetal plate. Inward bending of the vegetal plate first occurred at the position occupied by pigment cells, while the bending was not conspicuous in the ventral half of the blastopore. Rhodamine–phalloidin staining showed that actin filaments were abundant at the apical corticies of pigment cells. It was also found that the onset of gastrulation was considerably delayed in the NiCl2‐treated embryos, in which pigment cells were drastically reduced in number. It is notable that the NiCl2‐treated embryos began to gastrulate on schedule if they contained a number of pigment cells in spite of treatment. This shows that pigment cells are the bottle cells that trigger the onset of gastrulation. In the embryos devoid of pigment cells, a short stub‐like gut rudiment formed in a delayed fashion, and several secondary mesenchyme cells (SMC) appeared at the tip of the rudiment and elongated gradually until its tip reached the apical plate. This observation suggests that the SMC that pull the gut rudiment upward are not pigment cells but blastocoelar cells, because pigment cells change their fate to blastocoelar cells upon NiCl2‐treatment.
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期刊:
Development (Cambridge, England)
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