Biosorption study of copper and zinc by particles produced from silk sericin - alginate blend: evaluation of blend proportion and thermal cross-linking process in particles production
Biosorption study of copper and zinc by particles produced from silk sericin - alginate blend: evaluation of blend proportion and thermal cross-linking process in particles production
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DOI：
10.1016/j.jclepro.2015.05.067
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发表时间：
2016-11-20
影响因子：
11.1
通讯作者：
Carlos da Silva, Meuris Gurgel
中科院分区：
文献类型：
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作者：
da Silva, Thiago Lopes;da Silva Junior, Absolon Carvalho;Carlos da Silva, Meuris Gurgel
丝胶是一种存在于蚕丝（Bombyx mori）茧中的蛋白质，通常被丢弃在纺纱过程的废水中，而海藻酸盐是一种线性天然多糖，主要从褐藻中提取。这两种生物材料因其特性而受到关注。分离的丝胶蛋白结构性能较弱，但其强极性侧基易于交联、聚合和共混。混合物的使用改善了用蛋白质生产的材料的物理特性，并且藻酸盐的使用具有优势，因为它对多种阳离子具有亲和力。本文的目的是评估丝胶和海藻酸盐混合物产生的颗粒及其性能。采用胶凝技术，将混合物滴入 CaCl2 的水溶液和醇溶液中，用于生产丝胶溶液（2.5% w/V）中含有 1%、2% 和 3% w/V 海藻酸盐的颗粒。随后，在丝胶藻酸盐颗粒中进行了 40、100、125 和 150 摄氏度的热交联过程，并研究了这些颗粒对锌和铜金属的生物吸附过程。通过水溶性测量、扫描电子显微镜（SEM）图像、傅里叶变换红外光谱（STIR）光谱和吸附能力对数据进行评估。当颗粒在酒精溶液中产生时，其水溶性降低，这可能与 FTIT 分析证实的 β-折叠结构的形成有关。此外，还观察到当在醇溶液中产生颗粒时吸附容量增加。热交联过程提高了吸附能力直至100℃，Cu2+和Zn2+的还原值分别约为75%和65%。从温度 100 摄氏度开始，由于蛋白质/多糖的降解，所有颗粒的褐变程度都较高，溶解度增加，颗粒吸附能力下降。 (C) 2015 Elsevier Ltd. 保留所有权利。
Sericin is a protein present in the cocoon of silkworm silk (Bombyx mori), usually discarded in the effluent from the spinning process, and alginate is a linear natural polysaccharide, extracted mainly from brown algae. Both biomaterials have been attracting attention due to their properties. Sericin isolated present weak structural properties, but its strongly polar side groups enable easy cross-linking, polymerization and blending. The use of blends provides an improvement in the physical characteristics of the materials produced with the protein, and the use of alginate has an advantage because it has affinity for a variety of cations. The aim of this paper is to evaluate the particles produced from the blend between sericin and alginate and its properties. The gelation technique, dripping blends in aqueous and alcoholic solutions of CaCl2, was applied to produce particles with 1%, 2% and 3% w/V of alginate in sericin solution (2.5% w/V). Posteriorly, the thermal cross-linking process at 40, 100, 125 and 150 degrees C was performed in sericinalginate particles and the biosorption processes of these particles for zinc and copper metals were investigated. The evaluation of the data was carried out through the measurement of water solubility, Scanning Electron Microscopy (SEM) images, Fourier Transform Infrared Spectrometry (STIR) spectra and adsorption capacity. Water solubility of particles decreased when they were produced in alcoholic solution, which is probably related to the formation of beta-sheet structure confirmed by FTIT analyses. Also, it was observed that adsorption capacity increased when the particles were produced in alcohol solution. The thermal cross-linking process increased the adsorption capacity until 100 degrees C, with values around 75% and 65% of reduction of Cu2+ and Zn2+, respectively. From temperature 100 degrees C all particles got higher degree of browning with an increase of solubility and decrease of adsorption capacity by particles because of the degradation of protein/polysaccharide. (C) 2015 Elsevier Ltd. All rights reserved.