RIBONUCLEASE-H - AN ENZYME DEGRADING RNA MOIETY OF DNA-RNA HYBRIDS
RIBONUCLEASE-H - AN ENZYME DEGRADING RNA MOIETY OF DNA-RNA HYBRIDS
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DOI：
10.1111/j.1432-1033.1970.tb00287.x
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发表时间：
1970-01-01
期刊：
影响因子：
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通讯作者：
STEIN, H
中科院分区：
文献类型：
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作者：
HAUSEN, P;STEIN, H
小牛胸腺提取物含有一种酶，可降解 DNA-RNA 杂交体的 RNA 部分。描述了该酶的测定以及酶反应的几个特征。多种证据表明了该酶对 DNA-RNA 杂交体的特异性： 加热后，杂交体的 RNA 对这种酶的作用产生了抵抗力。 DNA-RNA 杂交体以外的核酸不会受到同等程度的影响。如果该酶与聚脱氧核糖腺苷酸杂交，则该酶会快速降解聚核糖苷酸，但如果它与聚核糖腺苷酸杂交则不会。双标记 DNA-RNA 杂交体的实验表明，酶会攻击杂交体的 RNA 部分，而不是 DNA。
Extracts from calf thymus contain an enzyme degrading the RNA moiety of DNA‐RNA hybrids. An assay for this enzyme and several characteristics of the enzyme reaction are described. The specificity of the enzyme for DNA‐RNA hybrids is indicated by several lines of evidence: The RNA of the hybrid is rendered resistant to the action of this enzyme upon heating. Nucleic acids other than DNA‐RNA hybrids are not affected to a comparable degree. The enzyme rapidly degrades polyribouridylic acid if it is hybridized to polydeoxyriboadenylic acid, but not if it is hybridized to polyriboadenylic acid. Experiments with double labelled DNA‐RNA hybrids show that specifically the RNA moiety of the hybrid and not the DNA is attacked by the enzyme.