Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Polη
Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Polη
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DOI：
10.1126/science.283.5404.1001
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发表时间：
1999-02-12
期刊：
影响因子：
56.9
通讯作者：
Prakash, L
中科院分区：
文献类型：
--
作者：
Johnson, RE;Prakash, S;Prakash, L
酿酒酵母的RAD 30基因是紫外线照射损伤的DNA的无错误复制后修复所必需的。在这里，RAD 30被证明编码一种DNA聚合酶，可以有效地复制过去的胸腺嘧啶-胸腺嘧啶顺式-顺式环丁烷二聚体，病变，通常阻止DNA聚合酶。当在体外与所有四种核苷酸一起孵育时，Rad 30结合了两个与胸腺嘧啶-胸腺嘧啶二聚体相对的腺嘌呤。Rad 30是第七种被描述的真核DNA聚合酶，因此被命名为DNA聚合酶eta。
The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here, RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine-thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase eta.