Loops Adjacent to Catalytic Region and Molecular Stability of Man1312
Loops Adjacent to Catalytic Region and Molecular Stability of Man1312
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邻近催化区域的环和 Man1312 的分子稳定性
DOI：
10.1007/s12010-016-2087-7
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发表时间：
2016-05
期刊：
影响因子：
--
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Zhou H;Yong J;Gao H;Yuan Z;Yang W;Tian Y;Wu Y
半纤维素是自然界中第二大多糖，可以通过包括甘露聚糖酶在内的多种酶转化为乙醇产物。甘露聚糖酶是一种重要的水解植物中富含的甘露糖多糖的酶。优化的甘露聚糖酶有助于改善转化过程，使该技术具有高效性和竞争力。本工作研究了与活性区相邻的环对Man 1312酶性质的影响。环6和10是与Man1312催化区相邻的两个环，并且对两个环进行缺失诱变和残基取代。位点S145、Q148、N244和S255的缺失和位点N146、S147、S156和T157的取代使酶的稳定性显著增加。四联体突变体ManD4I4结合了所有的突变，其最适温度和Tm值分别比Man1312高5 °C和4 °C。从我们的数据中，我们可以得出结论，环的酶是重要的设计诱变和获得改进的性质，特别是环邻近催化区的三级结构。在我们的实验中，在催化区附近的环上的残基缺失和取代使酶的性质得到显着改善。
Hemicelluloses are the second major polysaccharides in nature and can be converted to ethanol product by a variety of enzymes including mannanases. Mannanase is an important enzyme that hydrolyses mannose-containing polysaccharides which are abundant in plants. An optimized mannanase could help to improve conversion process and make the technology efficiently and competitively. In this work, the effects of loops adjacent to active region on enzymic properties of Man1312 were investigated. Loop 6 and 10 are two loops neighboring to Man1312 catalytic region, and deletion mutagenesis and residue substitution were performed on both loops. Deletion on sites S145, Q148, N244, and S255 and substitution on sites N146, S147, S156, and T157 gave significant increased stability to enzyme. The quadruplet mutant ManD4I4 combined all the mutations and had higher optimal temperature andTmvalue by 5 and 4 °C than Man1312, respectively. From our data, we are able to conclude the loops of enzymes are important to design mutagenesis and obtain improved properties, especially the loops neighboring to catalytic region from tertiary structure. In our experiment, residue deletion and substitution on loops neighboring to catalytic region made significant improvement on enzyme properties.
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DOI：
10.1007/s10295-003-0049-x
发表时间：
2003-05-01
影响因子：
3.4
作者：
Saha, BC
通讯作者：
Saha, BC
DOI：
--
发表时间：
2012
期刊：
--
影响因子：
--
作者：
S. Ding;Yu-San Liu;Y. Zeng;M. Himmel;J. O. Baker;E. Bayer
通讯作者：
S. Ding;Yu-San Liu;Y. Zeng;M. Himmel;J. O. Baker;E. Bayer
影响因子：
14.9
作者：
Tippana R;Xiao W;Myong S
通讯作者：
Myong S
DOI：
10.1093/bioinformatics/btq662
发表时间：
2011-02-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Benkert P;Biasini M;Schwede T
通讯作者：
Schwede T
影响因子：
4.1
作者：
Haiyan Zhou;Wenjiao Yang;Yun Tian;H. Peng;Yongyao Wu
通讯作者：
Haiyan Zhou;Wenjiao Yang;Yun Tian;H. Peng;Yongyao Wu