Structural basis for ribosome recruitment and manipulation by a viral IRES RNA
Structural basis for ribosome recruitment and manipulation by a viral IRES RNA
复制标题
DOI：
10.1126/science.1133281
复制
发表时间：
2006-12-01
期刊：
影响因子：
56.9
通讯作者：
Kieft, Jeffrey S.
中科院分区：
文献类型：
--
作者：
Pfingsten, Jennifer S.;Costantino, David A.;Kieft, Jeffrey S.
典型的帽依赖性翻译起始需要大量的蛋白质因子，这些因子在逐步组装过程中起作用。相反，内部核糖体进入位点（IRES）是顺式作用的RNA，在某些情况下，通过使用单一结构的RNA招募和激活核糖体来完全取代这些因子。在这里，我们提出的晶体结构的核糖体结合结构域从Dicistroviridae基因间区域IRES在3.1埃的分辨率，提供了一个视图的全RNA翻译起始装置的预折叠架构。将结构对接到IRES-核糖体复合物的冷冻电子显微镜重建中表明了通过动态IRES RNA进行核糖体操纵的模型。
Canonical cap - dependent translation initiation requires a large number of protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some cases completely supplant these factors by recruiting and activating the ribosome using a single structured RNA. Here we present the crystal structure of the ribosome-binding domain from a Dicistroviridae intergenic region IRES at 3.1 angstrom resolution, providing a view of the prefolded architecture of an all-RNA translation initiation apparatus. Docking of the structure into cryo - electron microscopy reconstructions of an IRES-ribosome complex suggests a model for ribosome manipulation by a dynamic IRES RNA.