Immunohistochemical distribution of S-100 protein in the cerebral cortex with regard to the cause of death in forensic autopsy
Immunohistochemical distribution of S-100 protein in the cerebral cortex with regard to the cause of death in forensic autopsy
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DOI：
10.1016/j.legalmed.2005.09.002
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发表时间：
2006-03-01
期刊：
影响因子：
1.5
通讯作者：
Maeda, Hitoshi
中科院分区：
文献类型：
--
作者：
Li, Dong-Ri;Zhu, Bao-Li;Maeda, Hitoshi
S-100 蛋白 (S100) 是一种酸性钙结合蛋白，在大脑中大量存在。本研究的目的是探讨法医尸检病例大脑皮层中 S100 的免疫组织化学分布及其与死因的关系及其与血清水平的关系。病例（n=286，死后3-48小时）包括致命性头部损伤（n=89）、其他钝器损伤引起的急性死亡（n=29）、锐器损伤（n=20）、窒息（n=29：勒颈/吊死，n=22；误吸，n=7）、溺水（n=22）、火灾死亡（n=68）、脑血管疾病（n=9）和急性心肌梗塞/缺血（AML n=20）。 S100 免疫阳性主要在胶质细胞中观察到，部分在神经元和髓磷脂中观察到。对于S100B免疫染色，胶质细胞和髓磷脂呈阳性，而神经元呈阴性。星形胶质细胞的阳性率取决于死亡原因，在因勒死/吊死和溺水而导致的急性死亡中显着较低，而在因受伤而导致的急性死亡中则略低。在迟发性头部损伤死亡和火灾死亡中经常观察到神经元和髓磷脂的阳性，这与头部受伤病例中星形胶质细胞的阳性呈反比关系。对于急性死亡的病例，星形胶质细胞中的 S100 阳性与血清 S100B 水平之间存在反比关系。这些观察结果表明，在致命性脑损伤期间，星形胶质细胞比神经元受到更快、更严重的损伤，从而导致血清 S 10013 水平升高。 (c) 2005 Elsevier Ireland Ltd. 保留所有权利。
S-100 protein (S100) is an acidic calcium-binding protein, which is abundantly found in the brain. The aim of the present study was to investigate the immunohistochemical distribution of S100 in the cerebral cortex in forensic autopsy cases with regard to the cause of death and relationship to its serum levels. The cases (n=286, 3-48 h postmortem) included fatal head injuries (n=89), acute death from other blunt injuries (n=29), sharp instrument injuries (n=20), asphyxiation (n=29: strangulation/hanging, n=22; aspiration, n=7), drownings (n=22), fire fatalities (n=68), cerebrovascular diseases (n=9) and acute myocardial infarction/ischemia (AML n=20). S100-immunopositivity was mainly observed in the gliacytes, in part, in the neurons and myelins. For S100B-immunostaining, the gliacytes and myelins were positive, whereas the neurons were negative. Positivity in astrocytes was dependent on the cause of death, and was significantly lower in acute deaths due to strangulation/hanging and drowning, and mildly low in those due to injuries. Positivity in neurons and myelins was frequently observed in delayed head injury deaths and fire fatalities, showing an inverse relationship with the positivity in astrocytes in head injury cases. For cases of acute death, there was an inverse relationship between S100-positivity in the astrocytes and the serum S100B level. These observations suggest that astrocytes are more rapidly and severely injured than neurons during fatal brain damage, thereby causing an elevation in the serum S 10013 level. (c) 2005 Elsevier Ireland Ltd. All rights reserved.