Synthesis of fluorescent carbon dots via microwave carbonization of citric acid in presence of tetraoctylammonium ion, and their application to cellular bioimaging
Synthesis of fluorescent carbon dots via microwave carbonization of citric acid in presence of tetraoctylammonium ion, and their application to cellular bioimaging
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DOI：
10.1007/s00604-015-1541-5
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发表时间：
2015-10-01
影响因子：
5.7
通讯作者：
Wu, Hui-Fen
中科院分区：
文献类型：
--
作者：
Bhaisare, Mukesh Lavkush;Abou Talib;Wu, Hui-Fen
以柠檬酸为原料，以四辛基溴化铵为催化剂，采用微波辅助合成法制备了凝胶状碳点。考察了四辛基溴化铵浓度对测定结果的影响。用紫外-可见光谱、X射线衍射仪、傅立叶变换红外光谱、荧光光谱和高分辨透射电子显微镜对所合成的碳点进行了表征。荧光从350 nm延伸到600 nm，对应的激发波长从300 nm到460 nm。量子收益率在0.11左右。一项细胞毒性研究表明，碳点具有细胞渗透性和生物相容性，这使得它们适合用于成像应用。这些点被用来通过这些点的蓝色荧光对HeLa细胞系进行成像。
A jelly-like form of carbon dots (C-dots) was prepared by microwave-assisted synthesis from citric acid in the presence of tetraoctylammonium bromide. The effect of the concentration of tetraoctylammonium bromide was examined. The synthesized carbon dots were characterized by UV-vis, XRD, FTIR, fluorescence and HR-TEM. Fluorescence extends from 350 to 600 nm, and the corresponding excitation wavelengths range from 300 to 460 nm. Quantum yields are at around 0.11. A cytotoxicity study showed carbon dots to be cell permeable and biocompatible which renders them appropriate for imaging applications. The dots were used to image HeLa cell lines via the blue fluorescence of the dots.