Distributions of allelic frequencies and haplotypes of two novel Y-chromosome STR in a Chinese population.
Distributions of allelic frequencies and haplotypes of two novel Y-chromosome STR in a Chinese population.
复制标题
中国人群中两种新型 Y 染色体 STR 的等位基因频率和单倍型分布。
DOI：
10.1520/jfs2002381
复制
发表时间：
2003
影响因子：
1.6
通讯作者：
Q. Ji
中科院分区：
文献类型：
--
作者：
Q. Zhu;J. Tang;Y. Z. Gao;B. Ying;Y. Hou;Y. Li;J. Wu;Q. Ji
中国成都的汉族男性。使用Chelex方法提取DNA（1）。PCR的反应体积为37.5 L，含有2-4 ng人类基因组DNA、200 M每种dNTP（Pharmacia，Sweden）、1.5 Taq聚合酶（Promega，麦迪逊，WI）、3.75 L 10缓冲液、1.5 mM Mg、1.6 g/mL BSA、0.3 M每种引物。扩增反应在Perkin Elmer 9600（Foster City，CA）中进行，其中在94°C预变性2分钟，然后是在94°C变性40秒、在58°C退火40秒和在72°C延伸25秒的35个循环。采用不连续缓冲液系统通过水平非变性聚丙烯酰胺凝胶电泳分析PCR产物，并通过银染色进行可视化（2）。群体遗传学和法医学资料按侯氏方法进行分析。完整的数据可以在http://www.legalmed上获得。org/dna/dys531.htm。引用
males of the Han ethnic group in Chengdu, China. DNA was extracted using the Chelex method (1). The reaction volume of PCR was 37.5 L, containing 2–4 ng human genome DNA, 200 M each dNTP (Pharmacia, Sweden), 1.5 Taq polymerase (Promega, Madison, WI), 3.75 L 10 buffer, Mg 1.5 mM, 1.6 g/mL BSA, 0.3 M each primer. Amplification reactions were carried out in a Perkin Elmer 9600 (Foster City, CA) with pre-denaturing for 2 min at 94°C, followed by 35 cycles of denaturing for 40 s at 94°C, annealing for 40 s at 58°C and extension for 25 s at 72°C. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data of population genetics and forensic science were analyzed according to Hou’s method (3). The complete data can be accessed at: http://www.legalmed. org/dna/dys531.htm. References