LOV-based optogenetic devices: light-driven modules to impart photoregulated control of cellular signaling.
LOV-based optogenetic devices: light-driven modules to impart photoregulated control of cellular signaling.
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基于LOV的光遗传设备：光驱动的模块，以赋予细胞信号传导的光心调节控制。
DOI：
10.3389/fmolb.2015.00018
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发表时间：
2015
影响因子：
5
通讯作者：
Zoltowski BD
中科院分区：
文献类型：
--
作者：
Pudasaini A;El-Arab KK;Zoltowski BD
光-氧-电压结构域蛋白家族广泛存在于生物学中，它们向信号转导结构域传递感觉反应。小型、光响应型LOV模块为构建光遗传工具提供了一个新的平台。目前，由于缺乏对光如何驱动蛋白质构象的变构变化以激活不同的信号转导结构域的了解，这些设备的设计和实现受到了部分阻碍。此外，LOV家族成员之间不同的光循环特性使具有快速开/关动力学的高灵敏度设备的结构变得复杂。在这篇综述中，我们讨论了LOV结构域研究的历史，主要侧重于调整LOV结构域化学和信号转导，以允许改进光遗传工具。
The Light-Oxygen-Voltage domain family of proteins is widespread in biology where they impart sensory responses to signal transduction domains. The small, light responsive LOV modules offer a novel platform for the construction of optogenetic tools. Currently, the design and implementation of these devices is partially hindered by a lack of understanding of how light drives allosteric changes in protein conformation to activate diverse signal transduction domains. Further, divergent photocycle properties amongst LOV family members complicate construction of highly sensitive devices with fast on/off kinetics. In the present review we discuss the history of LOV domain research with primary emphasis on tuning LOV domain chemistry and signal transduction to allow for improved optogenetic tools.