MicroRNA Expression in Melanocytic Nevi: The Usefulness of Formalin-Fixed, Paraffin-Embedded Material for miRNA Microarray Profiling
MicroRNA Expression in Melanocytic Nevi: The Usefulness of Formalin-Fixed, Paraffin-Embedded Material for miRNA Microarray Profiling
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DOI：
10.1038/jid.2008.347
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发表时间：
2009-05-01
影响因子：
6.5
通讯作者：
Drzewiecki, Krzysztof T.
中科院分区：
文献类型：
--
作者：
Glud, Martin;Klausen, Mikkel;Drzewiecki, Krzysztof T.
MicroRNAs(MiRNAs)是一种小的、非编码的RNA分子，调节细胞的分化、增殖和凋亡。MiRNAs以发育调节和组织特异性的方式表达。异常表达可能导致癌症等病理过程，因此miRNA可能作为生物标记物在临床环境中用于各种疾病的诊断。大多数miRNA图谱研究都使用了新鲜的组织样本。然而，在某些类型的癌症中，包括恶性黑色素瘤，很难从原发肿瘤获得新鲜材料，大多数手术标本是福尔马林固定和石蜡包埋(FFPE)。为了探索FFPE材料是否适合于黑素细胞病变的miRNA分析，我们比较了FFPE和新鲜冰冻样本中miRNA的表达模式，这些样本来自15个人的黑素细胞痣。从微阵列数据中，我们确定了84个在两种类型的样本中都表达的miRNA，并代表了黑色素细胞痣的miRNA图谱。我们的结果表明，配对的FFPE和新鲜的冷冻材料之间的miRNA表达具有高度的相关性(Spearman r值为0.80)。用定量RT-PCR对数据进行了进一步验证。总之，黑素细胞病变的FFPE标本适合作为miRNA微阵列图谱的来源。
MicroRNAs (miRNAs) are small, noncoding RNA molecules that regulate cellular differentiation, proliferation, and apoptosis. MiRNAs are expressed in a developmentally regulated and tissue-specific manner. Aberrant expression may contribute to pathological processes such as cancer, and miRNA may therefore serve as biomarkers that may be useful in a clinical environment for diagnosis of various diseases. Most miRNA profiling studies have used fresh tissue samples. However, in some types of cancer, including malignant melanoma, fresh material is difficult to obtain from primary tumors, and most surgical specimens are formalin fixed and paraffin embedded (FFPE). To explore whether FFPE material would be suitable for miRNA profiling in melanocytic lesions, we compared miRNA expression patterns in FFPE versus fresh frozen samples, obtained from 15 human melanocytic nevi. Out of microarray data, we identified 84 miRNAs that were expressed in both types of samples and represented an miRNA profile of melanocytic nevi. Our results showed a high correlation in miRNA expression (Spearman r-value of 0.80) between paired FFPE and fresh frozen material. The data were further validated by quantitative RT-PCR. In conclusion, FFPE specimens of melanocytic lesions are suitable as a source for miRNA microarray profiling.